产蛋下降鸭群强毒新城疫病毒的分离鉴定与分子特征  

Identfication and molecular characterization of a velogenic NDV isolated from egg drop ducks

作　　者：胡北侠[1] 王艳 杨少华[1] 许传田[1] 黄艳艳[1] 张秀美[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [2]六和饲料股份有限公司动保中心,山东青岛266109

出　　处：《中国兽医杂志》2012年第4期10-12,共3页Chinese Journal of Veterinary Medicine

基　　金：公益性行业(农业)科研专项(201003012)

摘　　要：从产蛋下降鸭群分离到两株新城疫病毒(NDV)(命名为Duck/China/SD6/2008和Duck/China/SD7/2009),经蚀斑纯化后测定其鸡胚平均死亡时间为77.6h,F蛋白裂解位点氨基酸序列为112-RRQKRF-117,符合强毒NDV的特征。根据F基因(374bp)对2个NDV分离株和30个NDV参考株进行基因分型结果表明,2个分离株均属于基因Ⅶ型。F基因和HN基因核苷酸序列同源性比较显示,2个鸭分离株与基因Ⅶ型NDV分离株同源性为94.1%～99.7%和94.4%～97.1%,与同期分离的鸡源NDV强毒株Chicken/China/SD4/2008同源性最高,分别为99.3%～99.5%和99.5%～99.7%,表明这两个鸭NDV毒株可能来源于感染NDV的鸡群。Two NDV isolates Duck/China/SD6/2008 and Duck/China/SD7/2009 were isolated from egg drop ducks.Both of them were determined as virulent strains with MDT of 77.6h,ICPI of 2.0 after plaque-purification.The amino-acid sequences of the fusion protein cleavage site in the two isolates both were 112-RRQKRF-117,which is a typical sequence of velogenic strains and is in agreement with the results of in-vivo pathogenicity tests.For genotyping,a phylogenetic tree based on nucleotides1-374 of the F gene was constructed using SD6/2008 and Duck/China/SD7/2009 and 30 other representative NDV sequences from GenBank.Phylogenetic analysis showed the isolate was of the genotype Ⅶ virus.The homology analysis of the nucleotide sequences of F and HN gene indicated that the two isolates have homologies of 94.1%～99.7%（F gene） and 94.4%～97.1%（HN gene） with genotypes Ⅶ strains,especially the NDV isolates Chicken/China/SD4/2008（HM748947）,with 99.3%～99.5% and 99.5%～99.7% identity.The results indicated that chicken flocks may be the source for the NDV isolates Duck/China/SD6/2008 and Duck/China/SD7/2009.

关 键 词：鸭 新城疫病毒 分离鉴定 序列分析 基因型 

分 类 号：S852.659.5[农业科学—基础兽医学]