HPLC法测定不同产地苦瓜根中苦瓜皂苷的含量  

Content Determination of Saponins in the Root of Momordica charantia from Different Areas by HPLC

作　　者：陈黎[1] 陈林[1] 申德堰[1] 何秀丽[1] 方建敏[1] 

机构地区：[1]湖北医药学院附属太和医院药学部,湖北十堰442000

出　　处：《中国药房》2012年第19期1784-1785,共2页China Pharmacy

摘　　要：目的:建立测定不同产地苦瓜根中苦瓜皂苷含量的方法。方法:采用高效液相色谱法。色谱柱为Gemini C18(250mm×4.6mm,5μm),流动相为水-甲醇(45:55),流速为1.0mL·min-1,检测波长为205nm,进样量为10μL。结果:苦瓜皂苷的检测浓度在0.051～1.020mg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9998);平均加样回收率为99.38%,RSD=1.99%(n=6)。结论:不同产地苦瓜根中苦瓜皂苷的含量有较大差别,可用此法较好地评价该品种的质量。OBJECTIVE：To determine the content of saponins in the root of Momordica charantia from different areas.METHODS：HPLC method was adopted.The determination was performed on Gemini C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of water-methanol （45：55） at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 205 nm,and the injection volume was 10 μL.RESULTS：The linear range of saponins was 0.051～1.020 mg·mL-1（r=0.999 8） with an average recovery of 99.38%（RSD=1.99%,n=6）.CONCLUSION：The content of saponins in the root of M.charantia from different areas varies greatly,and the method can be used for quality evaluation of the species.

关 键 词：苦瓜根 苦瓜皂苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]