荧光分光光度法测定硫酸阿米卡星的含量  

Determination of amikacin by fluorescence spectrophotometry

作　　者：李满秀[1] 刘凯凯[1] 石轶男[1] 

机构地区：[1]忻州师范学院化学系,忻州034000

出　　处：《分析试验室》2012年第5期44-46,共3页Chinese Journal of Analysis Laboratory

基　　金：山西省普通本科高等教育教学改革研究项目(晋教高(2009)44号);山西省高校高新技术产业化项目基金(2010020)资助

摘　　要：阿米卡星本身在激发波长295nm,发射波长367nm处有微弱荧光。它在NaNO2存在下,氨基亚硝化为硝基,然后在弱酸条件下水解为还原糖后与乙二胺在磷酸盐缓冲液中反应会产生强荧光物质。据此建立了阿米卡星含量测定的新方法。阿米卡星在2.0×10-5～5.0×10-6 mol/L的范围内与其产物荧光强度呈现良好的线性关系,相关系数r=09952,检出限为4.2×10-7mol/L。该方法用于市售硫酸阿米卡星注射液含量的测定。Amikacin has weak fluorescence at an excitation wavelength of 295nm,and an emission wavelength of 367nm.In the presence of sodium nitrite,amino-group was converted to nitryl.and then hydrolyzed in acidic conditions to produce reducing sugar.The reaction of reducing sugar with ethylenediamine in phosphate buffer solution will produce a strong fluorescent substance.A new method of measuring the content of amikacin was established according to this.A linear relationship between the concentration of amikacin in the range of 2.0×10-5-5.0×10-6 mol/L（r=09952） and fluorescence intensity was obtained with the detection limit of 4.2×10-7mol/L.This method was applied to the measurement of amikacin sulfate.

关 键 词：阿米卡星 荧光光度法 氨基糖苷类 

分 类 号：O657.3[理学—分析化学]