拟南芥叶绿体分裂突变体cpd111的基因定位与分析  

Genetic Mapping and Analysis of a Chloroplast Division Mutant cpd111 in Arabidopsis thaliana

作　　者：刘霞[1] 高岳芳[1] 石玉红[1] 沈鑫曌[1] 刘忠华[1] 高宏波[1,2] 

机构地区：[1]北京林业大学生命科学与技术学院,北京100083 [2]北京林业大学林木育种国家工程实验室,北京100083

出　　处：《植物学报》2012年第3期226-235,共10页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.31070162);北京市自然科学基金(No.5102022)

摘　　要：通过EMS(ethylmethane sulphonate)诱变从拟南芥(Arabidopsist haliana)突变体库中筛选到一个叶绿体分裂突变体chloroplas tdivision111(cpd111)。遗传学分析表明,该突变体的表型是单基因控制的隐性性状。与野生型相比,突变体植物细胞的叶绿体数量少,叶绿体形态和大小多样化,并且细胞体积与叶绿体数量之间无相关性。利用图位克隆的方法确定cpd111的突变基因为FtsZ1。进一步的分析表明,该突变影响FtsZ1基因mRNA的正常剪切和稳定性,使蛋白质翻译提前终止,最终导致叶绿体分裂异常。该工作为研究FtsZ1在叶绿体分裂中的作用提供了新的材料和线索。Ethyl-methane sulfonate mutagenesis revealed a chloroplast division mutant, chloroplast division111 （cpd111）, in Arabidopsis thaliana. Genetic analysis indicated that the mutant was controlled by a single recessive gene. Chloroplasts in the mutant were fewer in number and more heterogeneous in size than those in the wild type, The corre- lation between chloroplast number and cell size was not obvious in the mutant. Map-based cloning revealed a mutation in FtsZ1 responsible for the mutant phenotype. The mutation affected the splicing and stability of FtsZl mRNA, caused early translational termination of the protein and resulted in a chloroplast division defect in cpd111. We provide new materials and information for a better understanding of the function of FtsZ1 in chloroplast division.

关 键 词：拟南芥 叶绿体分裂 FtsZ1 图位克隆 mRNA剪切 

分 类 号：Q943[生物学—植物学]