五唇兰合子与原胚的分离  

Isolation of Zygotes and Proembryos in Doritis pulcherrima

作　　者：伍成厚[1,2] 李冬妹[3] 田惠桥[1] 

机构地区：[1]厦门大学生命科学学院,厦门361005 [2]广州市园林科学研究所,广州510405 [3]顺德职业技术学院,顺德528300

出　　处：《植物学报》2012年第3期286-291,共6页Chinese Bulletin of Botany

基　　金：广东省科技计划(No.2007B020801007);广州市科技计划(No.11A63030233)

摘　　要：分离合子和原胚可以为植物受精和胚胎发生提供很好的研究材料,因此具有重要意义。用酶解-振荡法分离出五唇兰(Doritis pulcherrima)受精后胚囊,然后以显微解剖获得合子和原胚。酶解液组成为0.7%–1.3%纤维素酶、0.6%–1.0%果胶酶和10%甘露醇,pH值为5.8,酶解时间0.5–3.0小时。在分离的早期原胚和接近成熟的球形胚中,珠孔端均有较发达的胚柄吸器。实验获得了对五唇兰胚胎发育的新认识,合子和原胚的成功分离也为进一步的细胞学和分子生物学研究打下基础。The isolation of zygotes and proembryos would provide invaluable material for the study of plant fertilization and embryogenesis. We isolated zygotes and proembryos by manual microdissection from Doritis pu/cherrima embryo sacs with only one layer of integument cells after pretreatment combining enzymatic maceration with shaking to the ovules. The enzymatic maceration was with 0.7%-1.3% cellulase, 0.6%-1.0% pectinase, and 10% mannitol, pH5.8, for one-half to 3 hours. Suspensor haustorium was discovered on the micropylar end from early proembryo to maturing spherical proembryo stages. The successful isolation of zygotes and proembryos in D. pu/cherrima provides a novel ap- proach to study embryogenesis and is a valuable experimental tool for further detailed research at the cellular and mo- lecular levels.

关 键 词：五唇兰 显微解剖 原胚 胚柄吸器 合子 

分 类 号：Q944.4[生物学—植物学]