热打击对培养人骨骼肌细胞的损伤和释放IL-6、TNF-α的影响  

Effect of gradient heat stress on skeletal muscle cells injuries and release of IL-6 and TNF-α

作　　者：潘志国[1] 耿焱[1] 刘灏[2] 万军[3] 刘云松[1] 苏磊[1] 

机构地区：[1]广州军区广州总医院重症医学科,全军热区创伤与组织修复重点实验室,510010 [2]南方医科大学中西医结合医院普外科,广州市510315 [3]解放军第458医院检验科,广州市510600

出　　处：《实用医学杂志》2012年第9期1405-1407,共3页The Journal of Practical Medicine

基　　金：广东省自然科学基金资助项目(编号:9151001002000021)

摘　　要：目的:研究梯度热打击对体外培养骨骼肌细胞的损伤和表达IL-6、TNF-α的影响。方法:利用CCK-8法对比各组不同时相细胞存活率和24h增殖率。采用ELISA法对比梯度热打击量对骨骼肌细胞释放IL-6和TNF-α的影响。结果:对比37℃对照组,热打击组各个时相细胞活力均下降(P<0.001),并呈时间及温度依赖关系。对比37℃对照组,39℃组7h时段及41℃和43℃组各时相增殖率显著下降(P<0.001),并呈时间及温度依赖关系。ELISA检查显示,热打击组IL-6和TNF-α的表达量较37℃对照组显著上升(P<0.001),41℃和43℃组较39℃组显著上升(P<0.01)。结论:热打击对骨骼肌细胞具有细胞毒效应,抑制骨骼肌细胞的增殖,并促进细胞因子IL-6和TNF-α的释放。Objective To investigate the effect of gradient heat stress on skeletal muscle cells injuries and release of IL-6 and TNF-α.Methods CCK-8 assay was used to investigate the difference of cell survival rate and 24 h proliferation rate among each group.ELISA was used to investigate the difference of IL-6 and TNF-α release in each group.Results In comparison with normal control group,the cell vitality of heat stress group were significantly decreased in each phase（P 0.001）,and this decrease appeared time-and thermal gradient-dependent.The proliferation rate of 7 h phase of 39℃ group and each phase of 41℃ and 43℃ groups were significantly decreased in comparison with normal control group（P 0.001）.This decrease was also time-and thermal gradient-dependent.The expression level of IL-6 and TNF-α of heat stress groups were significantly up-regulated in comparison with normal control group（P 0.001）.More IL-6 and TNF-α were released in cells of 41℃ and 43℃ groups than in cells of 39℃ group（P 0.01）.Conclusion Heat stress has cytotoxic effect on skeletal muscle cells,and can inhibit proliferation ability of these cells.Heat stress can promote the IL-6 and TNF-α release of these cells.

关 键 词：白细胞介素6 肿瘤坏死因子Α 热打击 骨骼肌细胞 细胞毒 细胞增殖 细胞因子 

分 类 号：R594.1[医药卫生—内科学]