高效氯氰菊酯亚慢性暴露对雌性幼鼠性腺作用  

Sub-chronic gonadal toxicity and its mechanism of β-cypermethrin in female immature mice

作　　者：何庆峰[1] 樊秀华[1] 张爱琳[1] 杜春生[1] 赵晨[1] 

机构地区：[1]天津农学院食品科学系食品质量与安全教研室,天津300384

出　　处：《中国公共卫生》2012年第5期627-628,共2页Chinese Journal of Public Health

基　　金：天津农学院科学研究发展基金(2008N009)

摘　　要：目的研究高效氯氰菊酯对雌性幼鼠性腺毒性作用及机理。方法将40只刚断乳雌性SPF级昆明小鼠按体重随机分为溶剂对照0.1、1.0、10 mg/kg高效氯氰菊酯组,皮下注射染毒,每天1次,连续染毒28 d,观察阴道开口时间,动情周期,测定血清中雌二醇,性腺(卵巢及子宫)湿重及系数,子宫内膜层雌激素受体(ER)表达。结果与对照组比较,高剂量高效氯氰菊酯组雌鼠动情期缩短,动情间期延长,子宫湿重(0.109±0.011)g增加(P<0.05),而各组子宫剥离重﹑子宫系数﹑血清雌二醇及子宫内膜腺体数量差异无统计学意义(P>0.05),中、高剂量组子宫内膜雌激素受体阳性表达率分别为(56.00±3.32)%,(29.00±5.58)%,明显低于对照组(72.8±3.84)%(P<0.05)。结论高效氯氰菊酯对幼年雌性小鼠生殖系统具有毒性作用,能降低子宫组织雌激素受体的表达。Objective To investigate the adverse effect and its mechanism of β-cypermethrin（β-CP） on gonads in female immature mice. Methods Fourty specific pathogen-free immature mice were randomly divided into a negative group（treated with earhnut oil） and three β-CP exposure groups（0.1,1.0,10 mg/kg body weight）.A daily subcutaneous exposure was conducted for 28 days.The time of vaginal open,estrous cycle,estrogen in serum,gonads（ovary and uterus） wet weight and coefficient,and the expression of estrogen receptor（ER） in endometrial were measured. Results Compared with the control group,the time of estrus shortened and the time of diestrus extended.The wet weight of uterus（0.109±0.011g） increased in the high dose group（P0.05）,but the coefficient and the blotted weight of uterus,the level of estrogen in serum and the sum of gonadal in endometrial were not significantly different among the groups（P0.05 for all）.In 1.0 and 10 mg/kg dose groups,the ratios of ER expression in endometrial was 56.00±3.32% and 29.00±5.58% and were lower than those of in the control group（P0.05）. Conclusion β-CP has gonadal subchronic toxicity and endocrine disrupting effect and can induce the expression of ER in uterus in female mice.

关 键 词：高效氯氰菊酯 亚慢性 性腺毒性 内分泌干扰物 

分 类 号：R114[医药卫生—卫生毒理学]