水稻(脱氧)胞苷酸脱氨酶基因的表达特性分析  

Expression Analysis of Genes Encoding Deoxycytidine/Cytidine Deaminases in Rice

作　　者：王学军[1] 康厚祥[2] 刘忠松[1] 王国梁[1,2] 

机构地区：[1]湖南农业大学作物基因工程湖南省重点实验室,湖南长沙410128 [2]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100193

出　　处：《中国水稻科学》2012年第3期261-266,共6页Chinese Journal of Rice Science

基　　金：湖南省自然科学基金资助项目(10JJ3042)

摘　　要：以水稻为材料,系统探讨了水稻中(脱氧)胞苷脱氨酶基因的组织表达特性和胁迫诱导表达特性。实时PCR结果表明,7个编码(脱氧)胞苷脱氨酶的基因在苗期水稻根、茎和叶中的表达具有组织特异性,但这些基因在水稻茎中的表达都低于根和叶中的表达;同时,这些基因胁迫诱导表达特性也不尽相同,只有Os07g0245100的表达同时受到低温、干旱、高盐和病害侵染等多种胁迫的诱导。因此,(脱氧)胞苷酸脱氨酶在水稻中可能通过调节体内嘧啶核苷的水平从而在水稻的环境胁迫应答反应中发挥重要调控作用。The phylogenetic relationship among the seven deoxycytidine/cytidine deaminase genes and the expression of these genes in different tissues and under different stresses were analyzed. Real-time PCR analysis showed that the seven genes had different expression characteristics in roots, stems and leaves with relative high expression levels in leaves and low expression levels in rice stems. Furthermore, the expression of most genes was stress-induced including chillness, drought, salination and disease infection, but only the expression of Os07g0245100 was induced by all four stresses. Our results demonstrated that the deoxycytidine/cytidine deaminase genes might play an important role in abiotic and biotic responses.

关 键 词：胁迫 (脱氧)胞苷酸脱氨酶 基因表达 水稻 

分 类 号：S511[农业科学—作物学]