稻曲病菌休眠与非休眠厚垣孢子胞壁多糖结构与组分  

Structure and Composition of Polysaccharide from Chlamydospore Wall in Ustiloginoidea virens

作　　者：王娜[1] 任佐华[1] 邓林伟[2] 陈娟芳[3] 刘二明[1] 

机构地区：[1]湖南农业大学生物安全科学技术学院,湖南长沙410128 [2]湖南农业大学生物科学技术学院,湖南长沙410128 [3]湖南农业大学东方科技学院,湖南长沙410128

出　　处：《中国水稻科学》2012年第3期356-360,共5页Chinese Journal of Rice Science

基　　金：国家自然科学基金资助项目(30871615)

摘　　要：为揭示稻曲病菌厚垣孢子壁与厚垣孢子内源性休眠的关系,以典型的休眠型(黑色)和非休眠型(黄色)稻曲病菌厚垣孢子为材料,用液氮研磨,超声破壁后,经复合酶-热水浸提-Sevag法提取胞壁多糖,采用紫外光谱、红外光谱、刚果红实验、气相色谱分析其多糖的结构和组分。结果表明,黑色、黄色厚垣孢子胞壁多糖均含β型吡喃糖苷键,具有三股螺旋构象,为主要由葡萄糖、半乳糖、甘露糖3种单糖组成的杂多糖,黄色厚垣孢子中含有少量的木糖;黑色厚垣孢子壁中葡萄糖、半乳糖和甘露糖的摩尔百分比为58.23∶25.32∶16.46,黄色厚垣孢子壁中葡萄糖、半乳糖、甘露糖和木糖的摩尔百分比为35.48∶30.58∶24.46∶9.45。这两种厚垣孢子壁多糖的组成和比例有明显差异,可能与厚垣孢子休眠有关。To reveal the relationship between endogenous dormancy mechanism of chlamydospores of U. virens and their walls, polysaccharide from cell walls of the dormant （black） and non-dormant （yellow） chlamydospores was obtained by complex enzyme-hot water extraction-Sevag after the chlamydospores wall breaking through liquid nitrogen grinding plus ultrasonic crushing technique, and structure and composition of cell wall polysaccharide were analyzed by uitraviolet absorption spectrum （UV spectrum） ,infrared absorption spectrum （IR spectrum）, Congo red test, and gas chromatography （GC）. The results showed that the polysaccharide from two kinds of the chlamydospores wall was acidic beteropolysaceharide and mainly consisted of the three monosaccharides, glucose, galactose and mannose, but the yellow chlamydospores wall still contained a small amount of xylose. Further, polysaccharide in the black chlamydospores wall, was composed of glucose, galactose, mannose with a molar percentage ratio of 58.23：25.32：16.46,while the yellow chlamydospore wall was composed of glucose, galactose, mannose and xylose, with a molar percentage ratio of 35. 48 = 30. 58 ： 24. 46 = 9. 45. The composition and composition ratios of monosaccharides in polysaccharide from the dormant and non-dormant chlamydospore walls obviously varied, which may be closely related to chlamydospore dormancy.

关 键 词：稻曲病菌 休眠 厚垣孢子壁 多糖 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]