显色底物磷脂酰肌醇的化学法制备及应用  

Chemical Preparation and Application of l-α-Phosphatidylinositol as a Chromogenic Substrate

作　　者：李琳[1,2,3] 吴清平[2] 张菊梅[2] 莫翠云[2] 郭伟鹏[2] 

机构地区：[1]中国科学院广州化学研究所,广东广州510650 [2]广东省微生物研究所广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室广东省华南应用微生物重点实验室-省部共建国家重点实验室培育基地,广东广州510070 [3]中国科学院研究生院,北京100049

出　　处：《精细化工》2012年第5期453-457,共5页Fine Chemicals

基　　金：广州市科技计划项目(2010U1-E00591;2010U1-E00611);广东省中国科学院全面战略合作专项项目(2010B090301037)~~

摘　　要：采用化学方法制备单核细胞增生李斯特菌(Listeria monocytogenes,LM)显色培养基底物磷脂酰肌醇(l-α-phosphatidylinositol,PI)。PI提纯过程中纯度和回收率是一对难以同时提高的矛盾因素,实验采用单一及复合溶剂萃取相结合的方法,考察了溶剂比、溶剂体积、碱性物质种类等条件对该矛盾因素的影响。经过优化研究,以氨水为碱性物质配制pH=8.0的甲醇溶液预处理后,采用V(正己烷)∶V(乙醇溶液)=1∶1.5时分离效果最佳。比较了纯化前后产物与标准品的微生物显色效果,结果证实了产品具有较好的应用价值。A chemical method was adopted to prepare the key substrate,l-α-phosphatidylinositol（PI） of Listeria monocytogenes（LM） chromogenic medium.The purity and the yield were two contradictory factors which could not be improved at the same time in the extraction of PI.The experiment in question combined single and mixed solvent methods to extract PI,during the process of which the influence of solvent ratio,solvent volume and the type of alkalinity substances on the purity and yield of the products were specifically investigated.The optimal conditions were as follows：the reaction to take place after the pretreatment of alkali methanol,at the solvent ratio of V（n-hexane）∶V（ethanol solution）=1∶1.5,using aqueous ammonia as alkalinity substance,and pH=8.0.Comparison of color development among the PI before and after purification and the product purchased from abroad shows that the product holds out a promising future for industrial application.

关 键 词：显色底物 单核增生性李斯特菌 磷脂酰肌醇 高效液相色谱 溶剂法 提纯技术 

分 类 号：TQ041.8[化学工程]