鸡新城疫病毒河北分离株F基因的分子特性分析  

Molecular characterization of fusion protein gene of Newcastle disease virus isolated from Hebei province

作　　者：马增军[1] 芮萍[1] 秦卓明[2] 李佩国[1] 左小磊[3] 韩东[4] 杨宗泽[1] 张艳英[1] 

机构地区：[1]河北科技师范学院,河北省预防兽医学重点实验室,河北昌黎066600 [2]山东农业科学院家禽研究所,山东济南250100 [3]石家庄市畜牧水产局,河北石家庄050000 [4]吉林大学畜牧兽医学院,吉林长春130062

出　　处：《中国兽医学报》2012年第5期650-653,共4页Chinese Journal of Veterinary Science

基　　金：河北省畜牧局计划项目(2005-3)

摘　　要：通过毒力测定、RT-PCR及F基因的序列测定与遗传进化分析,对2005-2008年从河北省部分地区的发病鸡群中分离到的10株新城疫病毒(NDV)进行了研究。各分离株经典毒力测定结果显示:MDT在37.6～54.4h之间,ICPI在1.71～2.0之间,IVPI值在2.16～2.8之间,均为新城疫病毒强毒株特征。F基因的序列测定表明,分离株之间的核苷酸序列具有77.4%～98.0%的同源性,与疫苗株Lasota的同源性为87.0%～98.9%,与国内标准强毒株F48E9同源性为89.7%～98.9%。推导其氨基酸序列分析表明,8个分离株的F蛋白的裂解位点氨基酸组成为112 R-R-Q-K-R-F117,具有NDV强毒株特征,与毒力测定结果相符,2个分离株的F蛋白的裂解位点氨基酸组成为112 G-R-Q-G-R-L117,与弱毒株特征相符。F基因分型和同源性比较显示:目前河北新城疫的流行以基因Ⅶ型为主(占70%),同时兼有基因Ⅱ型(占20%)和基因Ⅸ型(占10%)。Ten Newcastle disease virus（NDV） strains were isolated from chickens in the field outbreaks in some regions of Hebei province during 2004-2008.Assessment of the virulence by MDT,ICPI and IVPI,RT-PCR and sequence analysis of fusion protein gene were used to compare the properties of NDV isolates.The results indicated that MDT,ICPI and IVPI of the isolates were 37.6-54.4 h,1.71-2.0 and 2.16-2.8,respectively,indicating that the all NDV isolates were highly virulent.The results of nucleotide sequencing and phylogentic analysis of fusion protein gene showed that the ten strains shared homology from 77.4% to 98.0% among themselves,from 87.0% to 98.9% and from 89.7% to 98.9% with NDV Lasota,F48E8,respectively.The putative amino acid sequences of fusion protein at the cleavage sites of eight isolates were 112 R-R-Q-K-R-F117,with the motif characteristics of the virulent NDV strain;two isolates were 112 G-R-Q-G-R-L117,with the motif characteristics of the Lentongenic NDV strain.Phylogenetic analysis showed that the NDV epidemic belonged to 3 genotypes（II,Ⅶand Ⅸ） and the genotype VII（70%）was the major pathogeny causing the epizootic of ND in Hebei.

关 键 词：新城疫病毒 毒力 F基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]