1株鸡新城疫强毒株的分离鉴定及致病性  

Isolation and identification of high virulent avian Newcastle disease virus

作　　者：孙静[1] 刁有祥[1] 刘霞[1] 孙杰[1] 李建侠[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《中国兽医学报》2012年第5期658-661,709,共5页Chinese Journal of Veterinary Science

基　　金：山东省科技发展计划(2010GNC10912)

摘　　要：从山东省一发病率、死亡率高的肉鸡群中分离到1株病毒,经HA、HI试验和动物回归试验确定所分离的毒株为新城疫病毒,命名为新城疫LVCX株。按常规方法测得LVCX株的MDT、ICPI和IVPI分别为47.4h、1.975和2.85,鸡胚半数致死量LD50为10-9.8。F基因分析表明分离株LVCX为基因Ⅶ型,其多肽裂解位点为112-RRQKRF-117,表明LVCX株为新城疫病毒强毒株。该毒株F基因核苷酸同源性与近几年分离的基因Ⅶ型鸡源毒株同源性在93.5%～98.3%之间;与经典强毒株F48E9和Lasota株的核苷酸和氨基酸同源性分别为85.8%、91.3%和83.3%、88.4%;且与近几年分离的几株鹅源、鸭源核苷酸同源性也较高,在95.2%～96.5%之间。A strain virus isolated from the ill chicken in Shandong province was identified as Newcastle disease virus by HA,HI and neutralization of virus.The virus was named LVCX strain.The MDT,ICPI and IVPI for LVCX strain were 47.4 h,1.975 and 2.85,respectively.ELD50 of chicken embryos is 10-9.8.Amino acid sequence analyzing showed that the isolate was gene type Ⅶ.The FPCS of LVCX strain between positions 112 and 119 were 112-RRQKRF-117.The results indicated that LVCX isolate was virulent strain.The nucleotide homology of F gene is 93.5%-98.3% between LVCX strain and those of gene type Ⅶ viruses isolated from chicken.And the nucleotide homology of F gene is 95.2%-96.5% between LVCX strain and those of ND viruses isolated from ducks and gooses.The nucleotide homologies of F gene are respectively 85.8% and 83.3% between LVCX strain and F48E9 and Lasota strain.And the amino acid homologies of F gene are respectively 91.3% and 88.4% between LVCX strain and F48E9 and Lasota strain.

关 键 词：新城疫强毒 分离 鉴定 F基因 

分 类 号：S852.659.5[农业科学—基础兽医学]