aadA叶绿体转化烟草对根际微土壤生物的影响  

Effects of aadAtransplastomic tobacco on rhizosphere microbial communities in soil

作　　者：郭长虹[1] 刘庆国[1] 刘佳莉[1] 束永俊[1] 吕月萍[1] 余建平[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江省分子细胞遗传与遗传育种重点实验室,哈尔滨150025

出　　处：《哈尔滨工业大学学报》2012年第4期141-144,共4页Journal of Harbin Institute of Technology

基　　金：国家自然科学基金资助项目(30970552);黑龙江省高校科技创新团队研究计划项目(2010TD10);哈尔滨师范大学科技创新团队研究计划项目(KJTD-2011-2)

摘　　要：为研究叶绿体转基因烟草对土壤根际微生物的影响,以aadA叶绿体转基因烟草和对照烟草为实验材料,对苗期、花期、成熟期烟草根际细菌、放线菌及真菌的菌落数进行统计,利用Biolog法分析微生物群落功能多样性.结果表明:在相同生育期,aadA叶绿体转基因烟草与对照烟草相比,根际土壤细菌、放线菌及真菌数量均无显著差异;根际土壤微生物群落的代谢相似,转基因烟草对微生物群落功能的影响不显著.aadA叶绿体转基因烟草没有对土壤微生物数量和功能多样性产生显著的影响.To study the effects of aadA transplastomic tobacco （TA） on soil microbial communities, the quantity of bacteria, actinomycetes, and fungi was investigated at seedling stage, flowering stage, and senescing stage by plate cultivation of microorganism, and the functional diversity of rhizosphere microorganisms was de- tected by Biolog Ecoplate method. The results showed that there was no significant difference on the popula- tions of bacteria, actinomycetes, and fungi between aadA transplastomic tobacco and non-transgenic tobacco at same growing stage. The Biolog analysis indicated that the metabolism of rhizosphere microbial community were similar, aadA transplastomic tobacco didn＇ t affect the microbial community. In summary, aadA trans- plastomic tobacco had no significant effects on quantity and functional diversity of rhizosphere microorganisms in soil during one year growth.

关 键 词：叶绿体转化烟草 根际微生物 Biolog法 aadA基因 

分 类 号：S3[农业科学—农艺学]