菜蛾盘绒茧蜂滞育与非滞育预蛹过氧化氢、抗氧化酶及激素水平的比较  

Comparison of Hydrogen Peroxide,Antioxidant Enzymes and Hormones between Diapause and Non-diapause Prepupae of Cotesia vestalis

作　　者：郝仲萍[1] 袁忠勤[1] 张娟[1] 赵金瑞[1] 施祖华[1] 

机构地区：[1]浙江大学昆虫科学研究所,杭州310029

出　　处：《中国生物防治学报》2012年第2期165-169,共5页Chinese Journal of Biological Control

基　　金：国家重点基础研究发展计划项目(2009CB119005);国家自然科学基金项目(U0936601);农业部948项目(2011-G4);国家公益性行业(农业)科研专项(201103021)

摘　　要：为了研究过氧化氢与激素在菜蛾盘绒茧蜂Cotesia vestalis滞育过程中的作用,本文测定了滞育与非滞育预蛹过氧化氢的含量,3种保护酶(SOD、POD和CAT)的活性以及20E和JHⅢ的滴度,结果显示,滞育预蛹过氧化氢含量、SOD和POD活性以及20E滴度显著低于非滞育预蛹,而CAT活性与JHⅢ滴度则相反。推测高浓度的JHⅢ抑制20E的合成和释放,20E滴度的下降又通过调节SOD活性的下降和CAT活性的上升来降低过氧化氢的浓度,使菜蛾盘绒茧蜂滞育。In order to investigate the effects of hydrogen peroxide and hormones on diapause in Cotesia vestalis,hydrogen peroxide content,activities of three antioxidant enzymes（superoxide dismutase（SOD）,peroxidase（POD）,and catalase（CAT））,and contents of 20E and JH Ⅲ between diapause and non-diapause prepupae were compared.Results showed that the hydrogen peroxide content,SOD and POD activities and 20E content in diapause prepupae were significantly lower,while the CAT activity and JH Ⅲ content were significantly higher,when compared to those in non-diapause prepupae.We speculated that high concentrations of JH Ⅲ inhibited the synthesis and release of 20E,the latter reduced hydrogen peroxide content by decreasing SOD activity and increasing CAT activity,and finally caused prepupae of C.vestalis to enter diapause.

关 键 词：菜蛾盘绒茧蜂 过氧化氢 激素 抗氧化酶 滞育 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治] Q965[农业科学—植物保护]