一株银杏内生真菌的鉴定及其活性代谢产物研究  

Identification of an Endophytic Fungus of Ginkgo biloba TMSF169 and Its Antifungal Metabolites

作　　者：王国平[1,2] 王丽薇[2,3] 张亚磊[2,4] 王佳莹[1,2] 徐旭辉[5] 章初龙[1,2] 

机构地区：[1]浙江大化浙大生物药物研发中心,杭州310058 [2]浙江大学生物技术研究所,杭州310058 [3]杭州师范大学医药卫生管理学院,杭州310036 [4]浙江工业大学药学院,杭州310029 [5]浙江大学农药与环境毒理研究所,杭州310029

出　　处：《中国生物防治学报》2012年第2期226-234,共9页Chinese Journal of Biological Control

基　　金：公益性行业(农业)科研专项(200903052);杭州市创业种子基金(20102431K156)

摘　　要：药用植物内生真菌是一类重要的微生物资源,能代谢产生多种生物活性物质,本研究从浙江天目山自然保护区银杏Ginkgo biloba中分离获得1株活性菌株TMSF169。通过形态学和ITS rDNA序列分析,鉴定该菌株为团青霉Penicillium commune。发酵粗提物采用正相硅胶柱层析和Sephadex LH-20凝胶柱层析,以紫外光或碘蒸汽显迹,配合活性追踪,从菌株TMSF169发酵液中分离获得1个具抗真菌及除草活性的化合物TMSF169A,通过质谱和核磁共振波谱等技术将其结构鉴定为圆弧菌醛酸(cyclopaldic acid)。活性测试表明,该化合物具有抗菌活性,对鸭跖草Commelina communis,反枝苋Amaranthus retroflexus,马唐Digitaria sanguinalis、空心莲子草Alternanthera philoxeroides、水稻Oryza sativa等植物均具有致病毒性,对芝麻Sesamum indicum和大豆Glycine max不敏感。结果显示菌株TMSF169具有较好的开发应用价值。Endophytic fungi in medicinal plants are important sources for microorganisms which can produce a variety of bioactive compounds.In this study,an endophytic fungus TMSF169 with antifungal activity was isolated from Ginkgo biloba in Tianmushan Nature Reserve,Zhejiang province.TMSF169 was identified as Penicillium commune based on morphology and ITS rDNA sequence analysis.An antifungal and herbicidal compound TMSF169A was isolated and purified from the fermentation broth of TMSF169 through normal-phase silica gel column chromatography and Sephadex LH-20 gel column chromatography,traced by ultraviolet light or iodine vapor with bioassay-guided fractionation.TMSF169A was elucidated as cyclopaldic acid based on mass spectrometry and nuclear magnetic resonance spectroscopy.Antifungal activity assays showed that the compound had inhibitory to a series of plant pathogenic fungi.The metabolite was also pathogenic to Commelina communis,Digitaria sanguinalis,Amaranthus retroflexus,Alternanthera philoxeroides and Oryza sativa,but not sensitive to Sesamum indicum and Glycine max.All the results demonstrated that the strain TMSF169 deserves to develop further.

关 键 词：内生真菌 团青霉 活性代谢产物 除草活性 圆弧菌醛酸 

分 类 号：S482.292[农业科学—农药学] O658.1[农业科学—植物保护]