表达Harpin蛋白的芽孢杆菌工程菌防治水稻白叶枯病的研究  

Biocontrol Efficacy on Rice Bacterial Leaf Blight of Genetically Engineered Bacillus Expressing Harpin

作　　者：田大伟[1] 乔俊卿[1] 王伟舵[1] 伍辉军[1] 高学文[1] 

机构地区：[1]南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,南京210095

出　　处：《中国生物防治学报》2012年第2期250-254,共5页Chinese Journal of Biological Control

基　　金：国际科技合作项目(2009DFA32740);公益性行业(农业)科研专项(3-23);国家高技术研究发展计划(2006AA10Z172);国家大学生创新性实验计划(091030707);江苏高校优势学科建设工程资助项目

摘　　要：本文研究了生防促生芽孢杆菌OKB105、FZB42和以其为宿主菌的可外泌表达Harpin蛋白激发子的基因工程菌OKBHF、FZBHarpin对水稻白叶枯病的防治效果。菌株OKB105、FZB42、OKBHF和FZBHarpin温室防效分别为31.7%、23.6%、43.2%和56.7%;田间防效分别为29.8%、21.8%、40.4%和43.1%,表达Harpin蛋白的芽孢杆菌工程菌的防病效果显著高于出发菌株。两株生防芽孢杆菌及其工程菌对水稻均有良好的促生作用,特别是对水稻植株的生长促进明显,其中FZBHarpin使植株增高达15.86%。田间产量统计结果显示,OKBHF增产效果最显著,为14%,产量达8869 kg·hm-2。工程菌比出发菌株显著提高水稻抗病性和促进植物生长,在农业生产上具有应用的潜力。In this study,Bacillus OKB105、FZB42 and their genetically engineered bacteria OKBHF and FZBHarpin,which express and secret Harpin proteins,were determined for their biocontrol efficacy on rice bacterial leaf blight.Tests indicated that,control efficiacies of the four strains OKB105,FZB42,OKBHF and FZBHarpin were 31.7%,23.6%,43.2% and 56.7%,respectively,in greenhouse,while 29.8%,21.8%,40.4% and 43.1%,respectively,in field.Compared with patent strains,genetically engineered bacteria showed higher biocontrol efficacy and more significant plant growth-promoting capability,especially FZBHarpin which increased rice height by 15.86%.Results also indicated that yield of rice treated with OKBHF reached up to 8869 kg ·ha^- 1 by 14% increment.

关 键 词：芽孢杆菌 HARPIN蛋白 水稻白叶枯病 基因工程菌 生物防治 促生作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S435.111.47[农业科学—植物保护]