两株植物根际促生菌对番茄青枯病的生物防治效果评价  

Biological Control of Tomato Bacterial Wilt Caused by Ralstonia solanacearum with Erwinia persicinus RA2 and Bacillus pumilus WP8

作　　者：康贻军[1,2] 沈敏[1] 王欢莉[1] 赵庆新[1] 殷士学[2] 

机构地区：[1]盐城师范学院生命科学与技术学院,盐城224002 [2]扬州大学环境科学与工程学院,扬州225009

出　　处：《中国生物防治学报》2012年第2期255-261,共7页Chinese Journal of Biological Control

基　　金：国家自然科学基金(41101235;41071177);江苏省高校自然科学基金(10KJB210005);盐城师范学院博士科研启动基金

摘　　要：研究了Erwinia persicinus RA2和Bacillus pumilus WP8浸种和拌土处理对番茄青枯病的实际防治效果,及其对番茄根际微生物群落的影响。结果显示,两株菌都具有防治番茄青枯病的作用,并能不同程度地促进番茄幼苗生长。主要体现在显著提高番茄幼苗健株率,病原菌处理的健株率最低,仅为22.4%,而RA2和WP8浸种处理分别达68.9%和62.8%;促进幼苗地上部增高、增粗和根部生长,如WP8浸种处理的茎叶干重和根干重分别达到4.87 mg·株-1和35.69 mg·株-1,分别比病原菌对照提高110.82%和205.83%。浸种处理的促进效应明显优于拌土处理;还能在一定程度上提高土壤水稳性团聚体(>0.25 mm)比例,WP8浸种处理尤为明显,分别比空白对照和病原菌对照提高269.91%和156.88%。DGGE指纹图谱表明根际微生物群落受番茄种植的影响最大,其次是青枯病菌,而受这两种菌施用的影响最小。The effect of Erwinia persicinus RA2 and Bacillus pumilus WP8 with soaking and mixing soil treatments on tomato bacterial wilt and tomato rhizosphere bacterial community were studied.The results showed that both E.persicinus RA2 and B.pumilus WP8 could effectively control tomato bacterial wilt,and promote growth of tomato seedlings.Inoculation method with the seeds soaking was more effective than soil drench.The rates of healthy stand treated by seeds soaking were 68.9% and 62.8% respectively,significantly higher when compared to control and Ralstonia solanacearum QL-Rs1115 treatment;The dry biomass of stem and leaf and root reached 4.87 mg·strain^-1 and 35.69 mg·strain^-1 in B.pumilus WP8 soaking treatment,which were improved by 110.82% and 205.83% compared to R.solanacearum QL-Rs1115 treatment;Also,soil aggregate stability（〉0.25 mm） ratio were increased 269.91% and 156.88% compared to control and R.solanacearum QL-Rs1115 treatment respectively.DGGE fingerprinting indicated that the uses of E.persicinus RA2 and B.pumilus WP8 had minimal effects on rhizosphere bacterial community.

关 键 词：植物根际促生菌 番茄青枯病 生物防治 微生物群落 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.412.15[农业科学—植物保护]