胃和弓状核中obestatin在摄食行为调节中的作用  

The roles of obestatin in stomach and hypothalamic arcuate nucleus of mice on the regulation of feeding behaviour

作　　者：康冬梅[1] 赵翠平[1] 姚慧[1] 黄大可[2] 叶山东[3] 

机构地区：[1]安徽省立医院老年内分泌科,合肥230001 [2]安徽医科大学基础医学院综合实验室,合肥230032 [3]安徽省立医院内分泌科,合肥230001

出　　处：《安徽医科大学学报》2012年第5期508-510,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:070413104)

摘　　要：目的观察摄食行为改变对小鼠胃和下丘脑弓状核中obestatin表达的影响,探讨obestatin在摄食行为调节中的作用。方法 50只雄性昆明种小鼠随机分为A、B、C、D、E 5组,分别为饥饿0、24、48、72 h及饥饿72 h后再进食4 h。分别于饥饿不同时段和饥饿后再进食时观察各组小鼠体重的变化;采用免疫组化法检测各组小鼠胃组织和弓状核中obestatin表达水平的变化。结果与A组相比,B、C、D组小鼠体重均明显降低(P<0.01),E组小鼠体重增加(P<0.05)。与A组比较,余4组胃和弓状核obestatin表达均明显降低(P<0.05),B、C、D、E组差异无统计学意义。线性相关分析表明弓状核obestatin表达与处死时体重、胃组织obestatin呈正相关,与体重变化呈负相关。结论饥饿和饥饿后再进食状态胃组织和弓状核中obestatin表达有变化,提示obestatin可能参与了摄食行为的调节。Objective To study the effects of change of feeding behaviour（including fasting and refeeding） on the expression of obestatin in stomach and hypothalamic arcuate nucleus（ARC） of mice,to investigate the roles of obestatin on the regulation of food intake.Methods Fifty Kunming white male mice were divided into 5 groups in this study.Group A： fasted for 0 h,Group B： fasted for 24 h,Group C： fasted for 48 h,Group D： fasted for 72 h,Group E： refed for 4 h after 72 h fasted.The changes of the body weight in each group on different times of fasting and refeeding were obeserved.The expression of obestatin in the stomach and ARC were studied by the technique of immunohistochemistry.Results Compared with Group A,the body weight was significantly reduced in Group B,C and D,and increased in Group E（P〈0.05）.The expression of obestatin in the stomach and ARC in Group B showed no significant difference with Group E.Expressions of obestatin in the ARC and stomach correlated positively with the died body weights,and there were significant negative correlations with the variation of body weight.Conclusion Obestatin expressions are change in the stomach and ARC after fasting and refeeding,which shows that obestatin may participate in the regulation of feeding behavior.

关 键 词：OBESTATIN 饥饿 进食 胃 弓状核 

分 类 号：R333[医药卫生—人体生理学] R338.24[医药卫生—基础医学]