白芍总苷对糖尿病大鼠肾组织TLR2、TLR4/NF-κB信号通路调节的影响  

Regulation of total glucosides of paeony TLR2,4 / NF-κB signal pathway in the kidney from diabetic rats

作　　者：赵莉[1] 苏双全[1] 夏林[1] 胡梅芬[1] 吴永贵[1] 

机构地区：[1]安徽医科大学第一附属医院肾脏内科,合肥230022

出　　处：《安徽医科大学学报》2012年第5期518-522,共5页Acta Universitatis Medicinalis Anhui

基　　金：教育部高等学校博士学科点专项科研基金(编号:20093420110003)

摘　　要：目的研究白芍总苷(TGP)对糖尿病大鼠肾组织中Toll样受体2(TLR2)、Toll样受体4(TLR4)、核转录因子-κB(NF-κB-p-p65)表达的影响。方法将链脲佐菌素(STZ)诱导的糖尿病大鼠模型随机分为模型组、TGP组(50、100、200mg/kg.d),正常大鼠为对照组,8周后免疫组化法检测肾组织中TLR2、TLR4、NF-κB-p-p65的表达。结果模型组大鼠尿白蛋白排泄率(AER)明显高于对照组(P<0.01),TGP(50、100、200 mg/kg.d)给药8周后大鼠AER水平明显低于模型组(P<0.05,P<0.01)。模型组大鼠肾组织中TLR2、TLR4与NF-κB-p-p65表达明显高于对照组(P<0.01),TGP(50、100、200 mg/kg.d)给药8周肾小球TLR2表达与模型组相比差异无显著性,肾小管-间质TLR2表达明显低于模型组(P<0.01),TGP(50、100、200 mg/kg.d)给药8周肾小球与肾小管-间质TLR4、NF-κB-p-p65表达明显低于模型组(P<0.05,P<0.01)。结论 TGP可显著改善糖尿病大鼠早期肾脏损害,其机制与部分抑制糖尿病大鼠肾组织中TLR2、TLR4/NF-κB信号通路有关。Objective To investigate the effect of total glucosides of paeony （TGP） on the expression of toll-like receptor 2 （TLR2） , TLR4 and NF-KB-p-p65 in the kidney from experimental diabetic rat. Methods Diabetes rats were induced with streptozotocin（STZ） ,and TGP （50,100,200 mg/kg . d ） was orally administered once a day for 8 weeks. Normal rats served as control group. The expressions of TLR2, TLR4 and NF-KB-p-p65 were determined by immunohistochemical method in the kidney. Results Urinary albumin excretion rate （AER） in model group was markedly obviously higher than that in the control group（P 〈0.01 ） , AER in model group was markedly attenuated than that in TGP（50,100 and 200 mg/kg. d） treatment. Compared with control rats, the expressions of TLR2, TLR4 and NF-KB-p-p65 were significantly increased in the kidney. TGP （50,100 and 200 mg/kg. d） treatment could markedly reduce TLR2 expression in the tubulo-interstitium, TLR4 and NF-KB-p-p65 expression in the glomeruli and tubulo-interstitium. Conclusion TGP treatment can ameliorate early renal injury by inhibiting the overexpression of TLR2,4/ NF-κB signal pathway in the kidney from diabetic rats.

关 键 词：糖尿病肾病 TOLL样受体 核转录因子 白芍总苷 

分 类 号：R587.24[医药卫生—内分泌] R392.12[医药卫生—内科学]