梅毒血清固定患者外周血调节性T细胞／Th17细胞平衡的研究  

Balance between regulatory T cells and T helper 17 cells in peripheral blood of patients with serofast syphilis

作　　者：赵建斌[1] 张明海[1] 马杰[1] 刘百灵[1] 张晓燕[1] 李青[1] 杨雪萍[1] 

机构地区：[1]江苏省徐州市第一人民医院皮肤科,221002

出　　处：《中华皮肤科杂志》2012年第5期347-349,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金（30700718）;徐州市社会发展科技计划项目（XF10C057）

摘　　要：目的探讨梅毒血清固定患者外周血调节性T细胞（Treg细胞）和Th17细胞平衡的变化。方法梅毒血清固定患者26例，正常人对照23例，利用流式细胞仪分别检测外周血Treg细胞和Th17细胞的比例及相关特异性转录因子Foxp3和ROR-γt的定量表达情况，并进行相关性分析。结果梅毒血清固定患者外周血CD4^＋T细胞中，Treg细胞比例为33．28％4-11．84％，明显高于正常人对照组（22．13％±7．79％，P〈0．01）；Th17细胞比例（3．17％4-2．32％）显著低于正常人对照组（8．87％±2．00％，P〈0．01）；CD4＋T细胞内转录因子Foxp3的表达量（2994．86±1099．18）高于正常人对照组（2539．72±1086．96，P〈0．05），而ROR-γt的表达量（1473．12±752．20）低于正常人对照组（1778．344-388．13，P〈0．05）。在所有受试者外周血CD4＋T细胞中Foxp3和ROR-γt的表达呈负相关（r=-0．48，P〈0．01）。结论梅毒血清固定患者外周血存在Treg／Th17细胞平衡的异常。Objective To assess the balance between regulatory T （Treg） cells and T helper 17 （Th17） cells in patients with serofast syphilis. Methods Blood samples were collected from 26 patients with serofast syphilis and 23 normal human controls. Flow cytometry was performed to determine the proportion of Treg and Th17 cells in peripheral blood and to quantify the expression of specific transcription factors Foxp3 and ROR-Tt in CD4^＋ T cells of these subjects. Correlation analysis was carried out. Results The proportion of Treg cells in CD4＊ T cells in the patients with serofast syphilis was 33.28% ± 11.84%, significantly higher than that in the normal controls （22.13% ± 7.79%, P 〈 0.01）, while the proportion of Th17 cells was significantly lower in the patients than in the normal controls （3.17% ± 2.32% vs. 8.87% ± 2.00%, P 〈 0.01 ）. Enhanced expression of Foxp3 and attenuated expression of ROR-γt were observed in CD4^＋ T cells from the patients compared with the controls （2994.86 ± 1099.18 vs. 2539.72 ± 1086.96, P 〈 0.05; 1473.12± 752.20 vs. 1778.34 ±388.13, P 〈 0.05）. There was a negative correlation between the expression of Foxp3 and ROR-γt in CD4^＋ T cells from all the subjects （r = -0.481, P 〈 0.01）. Conclusion The Treg/Th17 cell balance is abnormal in peripheral blood of patients with serofast syphilis.

关 键 词：调节性T细胞 血清固定 细胞平衡 外周血 转录因子FOXP3 患者 梅毒 CD4+T细胞 

分 类 号：R759.1[医药卫生—皮肤病学与性病学]