桤木叶片中NO与硝酸盐还原系统关系的研究  

Study on Relationship between Nitric Oxide and Nitrate Reduction in Leaf of Alnus cremastogyne

作　　者：苏梦云[1] 吴祖洪 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400

出　　处：《林业科学研究》2000年第2期141-146,共6页Forest Research

基　　金：浙江省自然科学基金项目!"一氧化氮与植物氮代谢及信号传导酶之间关系的研究"(编号 :3972 73)的部分内容

摘　　要：在桤木生长期间 ,叶片中的一氧化氮 (NO)含量和一氧化氮合成酶 (NOS)活力随生长而变化 ,即生长初期水平较低 ,以后逐渐升高 ,生长高峰期达到最高值 ,随后在叶片开始衰老时下降。硝酸还原酶 (NR)和谷氨酰胺合成酶 (GS)的活力变化与 NOS活力的变化类似 ,但 NR活力高峰出现比 NOS早 ,而 GS活力高峰期在 NOS之后。用 NO处理 ,叶片中 NO含量和 NOS活力都有所增加 ,但 NR活力只在幼苗中表现增加 ,而在离体枝条的叶片中则下降。在 NO处理时供给枝条 0 .0 5mol· L-1KNO3 溶液 ,可明显提高叶片中的 NR活力。如只用 KNO3 溶液诱导处理 ,除 NR活力增加外 ,也能提高叶片中的 NO含量和 NOS活力。这表明 NO和 NOS与 NR。The nitric oxide (NO) content and nitric oxide synthase (NOS) activity in seedlings varied with the growing period of Alnus cremastogyne. In the initial stage,NO content and NOS activity were low. They gradually raised until the peak of the growth. When leaf began aged,the NO content and NOS activity turned to low level. The changes of nitrate reductase (NR) and glutamine synthase (GS) activities in growing period were similar to the NOS,but the maximum activity of NR appeared earlier than that of NOS. The former was in June and the latter was in July. GS maximum activity appeared in September. When leavies were directly exposed to NO (5～30 μmol·L -1 ),NO content and NOS activity appeared obvious increase. NR activity in seedling leaf (in vivo) raised,and in young tree leaf (in vitro) reduced. Treated with NO the nitrate reductase (NR) activity in branch’s leaf cultivated in KNO 3 solution (0.05 mol·L -1 ) obviously raised. If branch were directly cultivated in KNO 3 solution,NO content and NOS activity appeared increase except for NR activity. The results showed that there exist relationship between the level of NO and NOS,and growing of A. cremastogyne. NO related to nitrate reduction.

关 键 词：桤木 调节作用 NO NOS NR 硝酸盐还原系统 

分 类 号：S718.43[农业科学—林学]