不同因素对离体培养的银杏幼茎腋芽生长发育的影响  

Effects of Various Factors on the Growth and Development of Cultured Axillary Buds of Ginkgo biloba in vitro

作　　者：郝岗平[1] 杜希华[1] 尤勇[2] 侯福林[1] 范志强 张慧娟[1] 

机构地区：[1]山东师范大学生物系,山东济南250014 [2]中国林业科学研究院林业研究所,北京100091 [3]山东省林业厅种苗站,山东济南250014

出　　处：《林业科学研究》2000年第2期217-221,共5页Forest Research

基　　金：山东省科委基金资助项目! (编号 :940 31 0 0 1 )

摘　　要：The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.The stem of Ginkgo biloba were cultured on MS,modified MS and DCR basal medium,supplemented with various concentration of hormones—NAA,ZT (zeatin) and two types of nurture-CH (casein hydrolysate) and Ade (adenine). The aim was to study the effects of various basal medium,different hormones and nurtures on the growth and development of Ginkgo biloba stem. The result showed that the modified MS with CH 500 mg·L -1 was the best medium for inducing the development of axillary buds;the medium with NAA,ZT and Ade promoted axillary buds to be callus and inhibited them developing into shoots. The shoot rate was 89.3%. After cultured for 40 days,the clustered buds were emerged from axillary buds. The detached buds from clustered buds were cultured on modified MS basal medium or supplemented with CH 500 mg·L -1 . The shoots grew well,and the reproduction coefficient was 3. The shoots from apical buds were also reproduced,and the reproduction coefficient was 4. The shoots were induced to root on MS+NAA 0.1 mg·L -1 medium,and the rooting rate was 33.3%.

关 键 词：银杏 腋芽 生长发育 基本培养基 激素 离体培养 

分 类 号：S792.950.5[农业科学—林木遗传育种] S664.304.3[农业科学—林学]