两株鹅源H6N2亚型禽流感广东分离株的全序列分析及致病性研究  

Sequence analysis and pathogenicity of 2 H6N2 subtype AIV isolates from geese in Guangdong

作　　者：罗维玉[1,2] 胡永浩[1] 邓国华[2] 施建忠[2] 丁晴微[2] 王明芳[1,2] 张文亮[2] 陈化兰[2] 

机构地区：[1]甘肃农业大学,甘肃兰州730070 [2]中国农业科学院哈尔滨兽医研究所、兽医生物技术国家重点实验室/农业部动物流感重点实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2012年第5期345-349,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家重点实验室国家开放基金(SKLVBP201003);973计划(2011CB505101)

摘　　要：为了解禽流感病毒(AIV)的变异情况,本研究对我国禽流感监测期间分离鉴定的两株鹅源AIVA/Goose/Guangdong/362/2009(H6N2)(GD/362/09)与A/Goose/Guangdong/244/2010(H6N2)(GD/244/10)进行全基因序列的测定和分析,并进行其对SPF鸡和BALB/c小鼠的致病性试验。序列分析显示:HA裂解位点的序列为339PQIETR↓GLFG348,表明两株病毒均为低致病力AIV。HA和NA的核苷酸同源性分别为84.5%和98.9%,另外,序列分析结果显示,GD/244/10的PA、M基因分别与高致病性AIV(HPAIV)A/aquatic bird/Korea/w74/2005(H5N2)和A/duck/Hong Kong/140/1998(H5N1)的同源性最高,表明其内部基因来源复杂,可能与H5 HPAIV发生重组或有共同的来源。病毒对动物的致病性试验结果显示:两株病毒均不能在鸡体内有效复制,在小鼠的肺脏能够有效复制,但在小鼠的鼻甲内只能检测到GD/244/10。To study the variation of avian influenza virus （AIV）, two AIVs （GD/362/09 and GD/244/10） of H6 subtype isolated from goose during the epidemiological survey were sequenced and analyzed. The sequencing results showed that both the isolates had the HA cleavage site of 339pQIETR , GLFG, which was a characteristic structure of low pathogenic AIV, and the nucleotide homology of HA and NA gene between GD/362/09 and GD/244/10 were 84.5% and 98.9%, the PA and M gene of GD/244/10 share the highest homology with that of A/Aquatic bird/Korea/w74/2005 （H5N2） and A/Duck/Hong Kong/140/1998 （H5N1）, which indicated that it evolved from the same parent or reassortment had already occurred between the H6 and H5 subtype. Pathogenicity was examined by experimental infection of SPF chickens and BALB/c mice intranasally with 106 EID50/100 μL or 106 EID50/50 μL, respectively. The results showed that both the H6 A1Vs of goose origin failed to replicate in chickens, but they were able to replicate in mouse lung efficiently and only GD/244/10 was detected in mouse turbinate.

关 键 词：H6禽流感病毒 进化分析 抗原分析 致病性 

分 类 号：S852.65[农业科学—基础兽医学]