六种中药提取物对白血病细胞株的体外抑制作用研究  

Anti-tumor Effect of TanshinoneⅡA,Tetrandrine,Honokiol,Curcumin,Oridonin and Paeonol on Leukemia Cell Lines

作　　者：单卿卿[1] 龚玉萍[1] 郭勇[1] 林娟[1] 周睿卿[1] 杨曦[1] 

机构地区：[1]四川大学华西医院血液科,成都610041

出　　处：《四川大学学报（医学版）》2012年第3期362-366,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30770912);四川省科技厅社会公益项目(2008SZ0017);教育部回国留学人员启动资金(20071108-18-4)资助

摘　　要：目的研究中药有效成分丹参酮ⅡA、粉防己碱、和厚朴酚、姜黄素、冬凌草甲素和丹皮酚对白血病细胞株SUP-B15、K562、CEM、HL-60、NB4的抗增殖作用。方法采用改良MTT法测定6种中药有效成分作用于上述5种白血病细胞株96h后细胞增殖活性的变化,利用线性回归方法计算药物对细胞作用的半数抑制浓度(IC50)值。结果丹参酮ⅡA对SUP-B15、K562、CEM、HL-60、NB4均有一定的增殖抑制作用,但是对HL-60细胞株的抑制作用明显低于其它白血病细胞株。粉防己碱、和厚朴酚、姜黄素、冬凌草甲素对5种细胞株均有增殖抑制作用,且作用相似,抑制作用差异无统计学意义。丹皮酚对5种肿瘤细胞株的增殖有一定的影响,但作用最弱。结论粉防己碱、和厚朴酚、姜黄素、冬凌草甲素对5种白血病细胞株SUP-B15、K562、CEM、HL-60、NB4均有增殖抑制作用,且差异并无统计学意义,证明它们抗白血病作用具有广谱性。丹参酮ⅡA对SUP-B15、K562、CEM、NB4的增殖抑制作用明显优于HL-60细胞株,说明它的抗白血病作用在不同细胞株中有差异性。丹皮酚对5种白血病细胞株的作用最弱。Objective To study the anti-tumor effect of tanshinoneⅡA,tetrandrine,honokiol,curcumin,oridonin and paeonol on leukemia cell lines SUP-B15,K562,CEM,HL-60 and NB4.Methods The leukemia cell lines were exposed to the six Chinese herbal components for 96 hours.The proliferative inhibitory effects were detected with MTT and described by IC50 value.Results TanshinoneⅡA inhibited the proliferations of SUP-B15,K562,CEM,HL-60 and NB4 cell lines,with HL-60 showing the least impact.Tetrandrine,honokiol,curcumin and oridonin inhibited the proliferations of SUP-B15,K562,CEM,HL-60 and NB4 cell lines and there was no significant difference between the cell lines.Paeonol did not have significant inhibitory effect on leukemia cell lines.Conclusion Tetrandrine,honokiol,curcumin and oridonin inhibit the proliferation of five cell lines SUP-B15,K562,CEM,HL-60,NB4,and the effects are similar,which means that their anticancer effects are quite broad.TanshinoneⅡA has better anti-leukemia effects on SUP-B15,K562,CEM,NB4 than on HL-60.The effect of paeonol against leukemia cell lines is poor.

关 键 词：丹参酮ⅡA 粉防己碱 和厚朴酚 姜黄素 冬凌草甲素 丹皮酚 白血病细胞株 

分 类 号：R285.5[医药卫生—中药学]