小麦淀粉粒结合蛋白多态性及蛋白表达差异的研究  

Polymorphisms and Protein Expression Differences of Starch Granule Protein in Wheat

作　　者：冶婷[1] 李卫华[1] 李士磊[1] 王亮[1] 高欢欢[1] 

机构地区：[1]石河子大学农学院/新疆兵团绿洲生态农业重点实验室,新疆石河子832003

出　　处：《麦类作物学报》2012年第2期349-355,共7页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30860145);新疆兵团"十二五育种攻关"项目(2011BA002)

摘　　要：为了解小麦淀粉粒结合蛋白(Starch granule proteins,SGPs)的多样性及与淀粉品质性状的关系,对来自中国、墨西哥和埃及的149份春小麦材料淀粉粒结合蛋白进行SDS-PAGE电泳分析,探讨了淀粉粒结合蛋白多态性及表达差异。结果表明,供试材料的SGP组成存在多态性,149份材料中分离了10种主要的SGPs和56种组合带谱,其中SGP-g和SGP-e是小麦中出现频率最高的淀粉粒结合蛋白(出现频率均在90%以上),而绝大多数小麦材料(92.6%)中包含了7～9条SGPs条带。材料的地理来源与SGPs蛋白谱带聚类的结果并不完全一致。Starch granule proteins（SGP） is a trace protein which combined with wheat starch grain.Different molecular weight starch grains with myrosinase-binding protein may related to starch quality traits.To understand the diversity of starch-binding protein and its relationships with starch quality traits,149 spring wheat materials from China,Mexico and Egypt,were used for starch granule-bound proteins analysis by SDS-PAGE electrophoresis and cluster analysis,and differential expression of SGP among materials also was studied.The studies showed that the composition of SGP in the tested materials was polymorphic,10 major SGPs and 56 band combinations were detected among the 149 materials.Among which,SGP-g and SGP-e were found with the highest frequency in wheat starch-binding proteins（more than 90%）.The vast majority of the wheat materials（92.6%） contain 7~9 SGPs bands.Geographical origin of materials was not consistent with the clustering based SGPs protein bands.

关 键 词：小麦 淀粉粒结合蛋白 多态性 差异表达 

分 类 号：S512.1[农业科学—作物学] S331