原发性干燥综合征患者外周血B细胞FcγRIIb的表达及临床意义  

Expression of FcγR IIb on peripheral B cells and its clinical significance in patients with primary Sjogren＇s syndrome

作　　者：周士亮[1] 车楠[2] 陆智敏[2] 吴倩[1] 施冬艳[1] 陈京京[1] 李霞[1] 孙凌云[1] 

机构地区：[1]南京大学医学院附属鼓楼医院风湿免疫科,210008 [2]南京医科大学鼓楼临床医学院风湿免疫科

出　　处：《中华风湿病学杂志》2012年第5期292-295,共4页Chinese Journal of Rheumatology

基　　金：基金项目：国家自然科学基金面上项目（30972736,81072473,81172847）

摘　　要：目的探讨原发性干燥综合征（pSS）患者外周血B细胞FcγRⅡb的表达及其临床意义。方法流式细胞术检测19例pSS患者、15名健康对照外周血B细胞FcγRIIb的平均荧光强度（MFI）；酶联免疫吸附试验（ELISA）方法测定19例pss患者血清抗SSA、SSB抗体水平，统计学分析采用t检验、单因素方差分析、SNK—q检验及Pearson相关分析。结果pSS患者CD19＋CD27＋忆性B细胞亚群的百分率[（20．8±2．7）％，19例]显著低于健康对照组[（37．8±2．2）％，15名]（t=-4．002，P〈0．01）；活动期pSS患者外周血CD19＋CD27＋记忆性B细胞Fcγ／RⅡb的MFI[（74±8），13例]低于非活动期组[（132±11），6例]及健康对照组[（139±12），15名]（F=10．699，P〈0．01）；pSS患者外周血CD19＋CD27＋记忆性B细胞FcγRIIb的MFI与pSS疾病活动指数（SSDAI）呈负相关（r=-0．744，P=0．0003）；抗SSA、SSB抗体阳性组pSS患者CD19＋CD27记忆性B细胞FcγRⅡb的MFI[分别为（75±3），12例；（48±7），5例]显著低于抗SSA、SSB抗体阴性组[分别为（122±11），7例；（108±9），14例]（t分别为-4．336和-3．776，P均〈0．01）；抗SSA抗体阳性组Dss患者CD19＋CD27＋记忆性B细胞FcγRⅡb的MFI与抗SSA抗体滴度呈负相关（r=-0．685，P=0．014）。结论pSS患者活动期外周血记忆性B细胞FcγRIIb表达与SSDAI呈负相关，并与抗SSA抗体呈负相关。FcγRIIb表达异常可能在pSS免疫发病机制中起重要作用。Objective To investigate the expression of FcγR IIb on peripheral B cells and its clinical significance in primary Sjogren＇s syndrome （pSS）. Methods FcγR IIb expression on peripheral B cells from 19 pSS patients and 15 healthy controls was examined by flow cytometry. The levels of serum anti-SSA and SSB antibodies were determined using enzyme-linked immunosorbent assay （ELISA）. Data were analyzed with t test, one-way ANOVA, SNK-q test and Pearson＇s correlation test. Results The percentage of memory CD19＋CD27＋ B cell subpopulation was significantly lower in pSS patients [ （20.8±2.7）% ] when compared to normal controls [ （37.8 ±2.2）% ] （t =-4.002, P〈0.01）. The level of expression of FcγR IIb in active pSS memory CD19＋CD27＋ B cells [MFI（74±8）] was significantly reduced when compared to inactive [MFI（132± 11）] and normal controls [MFI （139±12）] （F=10.699, P〈0.01）. The level of expression of FcγR II b on memory CD19＋CD27＋ B cells from pSS patients was inversely correlated with Sj6gren＇s syndrome disease activity index （SSDAI） （r=-0.744, P=0.0003）. pSS patients with the serum anti-SSA/SSB antibodies positive group [ MFI（75±3 ）, （48±7）] displayed a lower expression of FcγR II b on memory CD19＋CD27＋ B cell than in patients with the serum anti-SSMSSB antibodies negative group [ MFI （ 122± 11 ）, （108±9） ] （t=-4.336 and -3.776 respectively, the P value of both tests were less than 0.01 ）. The level of expression of FcγR II b in the memory CD19＋CD27＋B cells of patients with active pSS was inversely correlated with anti-SSA antibody titers （r=-0.685, P=-0.014）. Conclusion The expression of Fcγ R II b on peripheral memory B cells from active pSS patients is inversely correlated with SSDAI and is also inversely associated with anti-SSA antibody levels. Decreased expression of FcγR II b might play an important role in the pathogenesis of pSS.

关 键 词：干燥综合征 B淋巴细胞 受体 IGG 

分 类 号：R593[医药卫生—内科学]