丙泊酚抑制过氧化氢诱导人红细胞衰亡  

Propofol inhibits eryptosis of human erythrocyte induced by hydrogen peroxide in vitro

作　　者：曹晨[1] 叶博[1] 魏国[1] 张国荣[1] 

机构地区：[1]安徽医科大学空军总医院临床学院

出　　处：《国际麻醉学与复苏杂志》2012年第5期320-323,共4页International Journal of Anesthesiology and Resuscitation

基　　金：全军十二五课题

摘　　要：目的 观察过氧化氢（hydrogen peroxide，H2O2）在体外诱导人红细胞磷脂酰丝氨酸（phosphatidvlserine，Ps）外露及前向散射值（forwardscatter，FSC）的变化，探讨丙泊酚对此的影响和机制。方法健康成年人红细胞制成2％悬液，分为5组：对照组（c组）、H202组（H组）、丙泊酚10μmol／L±H2O2组（P10±H组）、丙泊酚50斗mol/L±H2O2组（P50±H组）、丙泊酚100μmol／L±H2O2组（P100±H组）。以离子霉素、维生素E和英脱利匹特为阳性和阴性对照。各组H2O2的反应浓度均为200μmol／L，孵育1h后用流式细胞仪检测红细胞PS标记率及FSC。所得数据用SPSS软件统计处理。结果红细胞Ps标记率H组比C组（15．20±1．01）、（1．45±0．21）（氏0．001）明显增高，P50±H组（3．09±1．66）和P100±H组（1．68±0．28）均比H组（15．20±1．01）明显低（P〈0．001）。FSCH组比C组小（1768±9）、（1808±26）（辟0．047），P50±H组比H组明显恢复（1811±16）、（1768±9）（P=0．037）。结论H2O2能诱导人红细胞衰亡，丙泊酚对其有明显抑制作用，其机制在于丙泊酚有较强的抗氧化及清除自由基功能。Objective To explore the effects of propofol on eryptosis stimulated by hydrogen peroxide （H2O2）, which was analyzed by phosphatidylserine （PS） exposure at erythrocyte surface and forward scatter （FSC） of erythrocyte in vitro. Methods Human erythroeytes isolated from healthy volunteers were made to 2% suspension and divided into 5 groups： group C （ringer solution alone）, group H （H202 alone）, group P10±H （10 Ixmol/L of propofol and HzO：）, group P50±H （50 p, mol/L of propofol and H202） and group P100±H （100 Ixmol/L of propofol and H20：）. At the same time, Ionomycin, Vitamin E and Intralipid were used to establish positive and negative control. H2O2 was used at a concentration of 200μmol/L. Both erythrocyte PS exposure and FSC were determined by flow eytometry after 1 hour. Data were analyzed in One-Way ANOVA by SPSS. Results Compared to group C, H2O2 at 200 μmol/L significantly increased the PS exposure of erythrocyte （15.20±1.01） vs （1.45±0.21）（P〈0.001 ） and decreased the FSC （1 768±9） vs（l 808±26）（P〈0.05）, which were effectively improved by propofol at 50 μmol/L （15.20±1.01） vs（3.09±1.66） in PS exposure（P〈0.001）,（1 768±9） vs （1 811±16） in FSC（P〈0.05）. Conclusions H2Oz induces human erythrocyte eryptosis in vitro, which can be effectively attenuated by propofol because of it＇s strong antioxidant and free radical scavenging activity.

关 键 词：红细胞 磷脂酰丝氨酸 丙泊酚 过氧化氢 

分 类 号：R96[医药卫生—药理学]