大鼠脑脊液抽取的新方法  

A New Method for Extracting Cerebrospinal Fluid of Rat

作　　者：任长虹[1] 高明清[1,2] 曹金强[1,3] 李宁[1] Kevin K.W.Wang 吉训明[1] 

机构地区：[1]首都医科大学宣武医院,北京100053 [2]潍坊医学院附属医院,潍坊261031 [3]重庆医科大学附属第一医院,重庆400016 [4]Center of Innovative Research,Banyan Biomarkers Inc.USA

出　　处：《实验动物科学》2012年第1期61-62,68,共3页Laboratory Animal Science

基　　金：国家自然科学基金(No.30870854);北京市教委科技创新平台项目(PXM2011_014226_07_000070);北京市优秀人才基金D类(2011D005018000007)资助

摘　　要：目的建立简便易行的大鼠脑脊液提取的新方法。方法将大鼠头部固定与身体成大约135°角,在枕骨嵴下凹陷处进针大约0.5 cm抵达小脑延髓池,缓慢抽取大鼠脑脊液80～100μL,然后将针退出。结果成功获得脑脊液80～100μL。结论此种脑脊液提取方法简单易行。Objective To establish an effective,convenient method for extracting the cerebrospinal fluid（CSF） sample of rat.Method Place the rat in position that the head forms about 135°.Insert a needle through the foramen in the occipital crest into cisterna magna about 0.5 cm deep.Gently moving syringe plunger to collect 80~100 μL of CSF and withdraw the needle.Results 80~100 μL CSF samples were collected successfully.Conclusion The CSF sample collection was an effective,convenient method

关 键 词：大鼠 脑脊液 

分 类 号：Q955[生物学—动物学]