5种他汀类药物对鸡胚绒毛尿囊膜血管新生的影响  

Effects of five statins on angiogenesis in chicken chorioallantoic membrane model

作　　者：董海燕[1] 郑巧伟[1] 张李侠[1] 邓祥[1] 张迪[1] 封卫毅[1] 魏友霞[1] 

机构地区：[1]西安交通大学医学院第一附属医院,陕西西安710061

出　　处：《中国医院药学杂志》2012年第9期655-658,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金项目(<骨髓源性细胞中促肿瘤血管新生细胞亚群的研究>;编号:81071765);教育部留学归国人员基金项目(<骨髓源性细胞在药物影响血管新生过程的作用研究>;编号:第41批);陕西省中医药管理局中医药科研课题(<当归补血汤促血管新生作用的物质基础及机制研究>;编号:jc37)

摘　　要：目的:观察5种他汀类药物对鸡胚绒毛尿囊膜(CAM)血管新生的影响,探讨他汀类药物是否具有独立于血脂调节作用以外的促进血管新生作用。方法:制备CAM模型,将不同浓度他汀类药物、重组牛碱性成纤维细胞生长因子和生理盐水分别通过载体加到CAM上,观察CAM特异性血管生长情况及血管数目变化。结果:不同浓度辛伐他汀(0.02,0.1,0.5,2.5,12.5μmol.L-1)中,当辛伐他汀浓度为0.1μmol.L-1时,CAM血管生成数量明显高于生理盐水对照组(P<0.05),与阳性对照组比较无统计学意义(P>0.05);而不同他汀类药物,即瑞舒伐他汀组(0.025μmol.L-1)、阿托伐他汀组(0.05μmol.L-1)、普伐他汀组(0.2μmol.L-1)、氟伐他汀组(0.4μmol.L-1),CAM血管生成数均明显高于生理盐水组(P<0.05),与阳性对照组比较无统计学意义(P>0.05)。结论:常用5种他汀类药物均有促进CAM血管新生作用;不同浓度辛伐他汀对CAM的作用不同。提示他汀类药物促进血管新生的作用可能与药物剂量有关。OBJECTIVE To observe the effects of five statins on the growth of vascularization in chicken chorioallantoic membrane （CAM） model, and to investigate the pro-angiogenic activity of statins independent of regulation of blood lipids. METHODS CAM model was employed to test the proor anti-angiogenic activities of simvastatin, pravastatin, atorvastatin, fluvastatin and rosuvastatin. The growth and the number of blood vessels were measured. RESULTS Under the conditions of 0.1 μmol. L- 1 of simvastatin, but not in the concentrations 0. 02,0. 5,2. 5,12.5 μmol. L- 1 of simvastatin, blood vessel density on CAM was more than that in the negative control group （P〈0. 05） significantly, and no significant difference was seen as compared with the positive control group （P〉0. 05）. The blood vessel densities under the concertrations,which were set up according to the concentration in plasma after an clinical equivalent oral dosage in rosuvastatin 0. 025μmol. L- 1, atorvastatin 0.05 μmol.L-1, pravastatin 0.2 μmol. L-1, fluvastatin 0. 4 μmol. L- 1, were more than that in the negative control group （P〈0.05） significantly,and there was no significant difference as compared with the positive control group （P〉0.05）. CONCLUSION

关 键 词：他汀类药物 鸡胚绒毛尿囊膜 血管新生 

分 类 号：R285[医药卫生—中药学]