基于CD14及IL-1β变化探讨清热化湿颗粒剂干预慢性糜烂性胃炎临床研究  

Effect of Qingre Huashi Granules on chronic erosive gastritis based on the changes of CD14 and IL-1β

作　　者：张涛[1] 陈远能[1] 张保静[1] 黄适[1] 宋杰[1] 

机构地区：[1]广西中医学院附属瑞康医院,广西南宁530011

出　　处：《中成药》2012年第5期787-790,共4页Chinese Traditional Patent Medicine

基　　金：广西自治区自然基金课题(2010jjA40346)

摘　　要：目的基于治疗前、后CD14、IL-1β以及中医证侯变化,主客观指标合参,客观评价清热化湿颗粒剂(广藿香、厚朴、茯苓、法半夏、苦杏仁、薏苡仁、淡竹叶、白豆蔻、泽泻、蒲公英、丁香)干预慢性糜烂性胃炎的临床疗效及其可能机制。方法 60例符合诊断标准、纳入标准慢性糜烂性胃炎患者予清热化湿方干预4周。详细记录治疗前、后中医证侯变化;应用免疫组化及Real-time PCR技术检测胃黏膜及血清CD14、IL-1β变化。结果清热化湿类方药治疗慢性糜烂性胃炎总有效率为76.66%,治疗前慢性糜烂性胃炎患者CD14、IL-1β蛋白及其mRNA表达呈上升趋势;治疗后,患者血浆CD14、IL-1β蛋白及其mRNA表达呈下降趋势,与治疗前比较有统计学意义(P<0.05)。结论清热化湿方可能通过调控CD14、IL-1β表达,明确缓解慢性糜烂性胃炎的效用,值得临床推广应用。AIM To study the mechanism of Qingre Huashi Granules（Pogostemonis Herba,Magnoliae officinalis Cortex,Poria,Pinelliae Rhizoma praeparatum,Armeniacae Semen amarum,Coicis Semen,Lophatheri Herba,Amomi cardamomi Fructus,Alismatis Rhizoma,Taraxaci Herba,Caryophylli Flos） interfering with chronic erosive gastritis by observing the TCM syndrome and the changes of CD14 and IL-1β.METHODS Qingre Huashi Granules were administrated to sixty CEG patients for 4 weeks.The changes of clinical manifestations were documented before and after treatment.The expression of CD14 and IL-1β was assayed by IHC and Real-time PCR.RESULTS The total effective rate of Qingre Huashi Granules in treating CEG was 76.66%.Before treatment,the mRNA and protein expression of CD14 and IL-1β was on the rise.After treatment,the expression of CD14 and IL-1β mRNA and protein was on the decline.There was a significant difference in the expression of CD14 and IL-1β before and after treatment（P0.05）.CONCLUSION Qingre Huashi Granules exert a detectable effect on alleviating chronic erosive gastritis by regulating the expression of CD14 and IL-1β.

关 键 词：CD14 IL-1Β 清热化湿颗粒剂 慢性糜烂性胃炎 

分 类 号：R287[医药卫生—中药学]