HPLC法测定复方苦参洗剂中盐酸小檗碱和黄芩苷  

Determination of berberine hydrochloride and baicalin in Compound Kushen Lotions by HPLC

作　　者：吴雪丹 丁宝月[2] 傅应华[2] 

机构地区：[1]浙江省象山县第一人民医院,浙江象山315700 [2]嘉兴学院医学院,浙江嘉兴314001

出　　处：《中成药》2012年第5期868-871,共4页Chinese Traditional Patent Medicine

摘　　要：目的采用HPLC法建立复方苦参洗剂(苦参、黄柏、蛇床子、百部、黄芩等)中盐酸小檗碱和黄芩苷测定方法。方法色谱柱为Agilent XDB-C18(25 cm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(每100 mL中含辛烷磺酸钠0.1 g),梯度洗脱,体积流量1.0 mL/min,检测波长280 nm,进样量20μL,柱温30℃。结果盐酸小檗碱在3.3～24.4μg/mL呈良好线性关系,黄芩苷在18.0～135.1μg/mL呈良好线性关系,平均回收率(n=6)分别为102.3%和101.6%;精密度试验RSD分别为2.55%和2.31%。结论本法简便,结果准确,能同时定量测定2种成分。AIM To establish a RP-HPLC for simultaneously determining berberine hydrochloride and baicalin in Compound Kushen Lotions（Sophorae flavescentis Radix,Phellodendri chinensis Cortex,Cnidii Fructus,Stemonae Radix,Scutellariae Radix,etc.）.METHODS The RP-HPLC method was established.The sample was separated and analyted on Agilent XDB-C18（25 cm×4.6 mm,5 μm） column,and acetonitrile-water（containing 0.1% phosphoric acid,0.2% sodium octanesulfonate）（20∶ 80） was used as mobile phase,gradient elution for 25 min.The flow rate was maintained at 1.0 mL/min,and the detective wavelength was set at 280 nm.The injection volume was 20 μL,and the column temperture was maintained at 30 ℃.RESULTS The calibration curve was linear in the ranges of 18.0-135.1 μg/mL for baicalin（r =0.999 8） and of 3.3-24.4 μg/mL for berberine hydrochloride（r =0.999 8）.The average recoveries（n=6） of sample were 101.6% and 102.3%,and the RSDs were 2.31% and 2.55%,respectively.CONCLUSION This method is simple,accurate,reproducible,and can be used for quantitative control of this preparation.

关 键 词：复方苦参洗剂 盐酸小檗碱 黄芩苷 黄柏 黄芩 HPLC 

分 类 号：R927.2[医药卫生—药学]