大鼠血浆中抗精神分裂症候选新药SIPI6360立体异构体的LC-MS/MS法测定  

Determination of the Stereoisomers of SIPI6360 in Rat Plasma by LC-MS/MS

作　　者：周一萌[1] 孙杰[2] 石劲敏[2] 唐黎明[2] 林梅[2] 周斌[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,上海200040 [2]上海市食品药品检验所,上海201203

出　　处：《中国医药工业杂志》2012年第5期368-371,共4页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项资助(2009ZX09103-007;2009ZX09103-048)

摘　　要：建立了液相色谱-串联质谱法拆分并测定大鼠血浆中SIPI6360的立体异构体。采用Daicel Chiralpak IA手性柱,以甲醇为流动相,采用ESI源正离子模式、多反应监测进行定量分析。结果显示,(R)-(+)-SIPI6360与(S)-(-)-SIPI6360立体异构体分离良好,在0.5～100 ng/ml浓度范围内线性关系良好。方法回收率为94.0%～102.8%,批内和批间RSD小于5.2%和11.4%。A LC-MS/MS method was established for the determination of the stereoisomers of SIPI6360 in rat plasma.A Daicel Chiralpak IA chiral column was used,with methanol as the mobile phase.A tandem mass spectrometric detection was conducted using multiple reaction monitoring（MRM） under positive ionization mode with an electrospray ionization（ESI） interface.The（R）-（＋）-form and（S）-（-）-form were well separated,and the calibration curves were linear in the concentration range of 0.5-100 ng/ml.The method recoveries were 94.0%-102.8%,with intra-and inter-batch RSDs below 5.2% and 11.4%.

关 键 词：SIPI6360 精神分裂症 立体异构体 液相色谱-串联质谱 测定 

分 类 号：R969[医药卫生—药理学] O657.72[医药卫生—药学]