5-氨基酮戊酸的柱前衍生化HPLC-荧光法测定  

Determination of 5-Aminolevulinic Acid by Pre-column Derivatization HPLC-FD

作　　者：石磊[1,2] 赵锋[3] 栾瀚森[3] 王浩[3] 王宏伟[1,2] 

机构地区：[1]安徽医科大学上海皮肤病临床学院 [2]上海市皮肤病医院,上海200050 [3]中国医药工业研究总院药物制剂国家工程研究中心,上海201203

出　　处：《中国医药工业杂志》2012年第5期377-380,共4页Chinese Journal of Pharmaceuticals

基　　金：上海市自然科学基金项目(11ZR1432800)

摘　　要：建立了柱前衍生化HPLC-荧光法测定5-氨基酮戊酸。以荧光胺为荧光衍生化试剂,使用C18柱,以乙腈-0.1%三氟乙酸溶液(30︰70)为流动相,激发波长和发射波长为398和480 nm。5-氨基酮戊酸在0.047 5～47.5 g/ml浓度范围内线性关系良好。回收率为99.2%,RSD为1.46%。A pre-column derivatization HPLC-FD method was established for the determination of 5-aminolevulinic acid.Fluorescamine was used as the derivatizing agent.A C18 column was used with the mobile phase of acetonitrile-0.1% trifluoroacetic acid solution（30︰70）.The excitation and emission wavelengths were 398 and 480 nm.The calibration curve of 5-aminolevulinic acid was linear in the concentration range of 0.047 5-47.5 μg/ml.The average recovery was 99.2%,with RSD of 1.46%.

关 键 词：5-氨基酮戊酸 荧光胺 高效液相色谱 柱前衍生化 荧光检测 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]