基于氧化石墨烯淬灭效应的DNA传感器特异性检测三聚氰胺  

A specific DNA sensor to detect melamine using graphene oxide

作　　者：李静[1] 裴昊[1] 李凡[1] 王丽华[1] 樊春海[1] 

机构地区：[1]中国科学院上海应用物理研究所,上海201800

出　　处：《核技术》2012年第5期386-389,共4页Nuclear Techniques

摘　　要：三聚氰胺(Melamine)在食品中的违规添加造成了严重的社会后果,引起了人们对三聚氰胺及其衍生物的普遍关注。本文利用氧化石墨烯(GO)独特的淬灭效应,以及GO与DNA和三聚氰胺相互作用的差异,建立了一种简便快速、特异性好的三聚氰胺检测方法。GO吸附荧光标记寡核苷酸(Fam-DNA)并导致荧光淬灭,得到低荧光的复合物,加入三聚氰胺后,GO与三聚氰胺的强相互作用导致DNA从GO表面脱落下来,荧光得到恢复。加入对照样品葡萄糖、麦芽糖、果糖等,荧光值没有明显升高。相比于以往检测三聚氰胺的方法,该方法操作简便、成本低廉、特异性好,适用于大量样品的快速初步筛选。The addition of melamine into food caused serious social problems,hence the need of an easy way of detecting melamine and its derivatives.In this paper,we propose a new method based on the unique quenching effect of graphene oxide（GO）.The difference of GO interaction with DNA and melamine is the basis of selective detection of melamine.GO adsorbs DNA and efficiently quenches the fluorescence of labeled fluorophores.The stronger interaction of melamine with GO than DNA leads to a competitive displacement of DNA out of the surface of GO,then a significant fluorescence increase appears.As a control,the glucose,maltose,and fructose do not cause significant fluorescence increase.This method is simple,inexpensive,sensitive and selective.It is suitable for rapid screening of large quantities of samples.

关 键 词：三聚氰胺 氧化石墨烯 寡核苷酸(Fam-DNA) 特异性检测 

分 类 号：Q61[生物学—生物物理学]