大孔吸附树脂富集沙枣花中总黄酮的工艺研究  

Study on the Enrichment Technology of Total Flavonoids in Elaeagnus Angustifolia Flower by Macroporous Adsorption Resin

作　　者：徐泉[1] 王培先[2] 程芬[3] 王妍[1] 

机构地区：[1]宁夏医科大学基础医学院,银川750004 [2]山东省胶南市人民医院,山东胶南266400 [3]西南交通大学生命科学与工程学院,成都610031

出　　处：《中国药学杂志》2012年第9期673-678,共6页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(21062015)

摘　　要：目的筛选适合分离和纯化沙枣花总黄酮的大孔吸附树脂并确立纯化工艺参数。方法以大孔吸附树脂吸附率及解吸率为指标,筛选出富集沙枣花总黄酮的最佳树脂,并通过单因素考察该树脂分离、纯化沙枣花总黄酮的最佳工艺条件。结果 DA201树脂对沙枣花总黄酮有良好的吸附分离性能。其具体工艺条件为:树脂柱径高比为1∶8、控制上样流速为0.5mL.min-1、上样液浓度20 mg.mL-1、调pH为4、洗脱流速为0.5 mL.min-1、最大上样为4.5 BV、洗脱剂浓度80%、洗脱剂用量12 BV。结论 DA201型树脂在所确定的工艺条件下,纯化沙枣花总黄酮效果良好,总黄酮得率达到85.23%,总黄酮纯度达到了14.95%,比粗提样品纯度(4.901%)提高了3倍。OBJECTIVE To screen the best macroporous adsorptive resin for separation and purification of flower Elaeagnus an- gustifolia flavonoids and to establish the purification process parameters. METHODS Using macroporous adsorption resin adsorption rate and desorption rate as the indexes, the best resin was screened out for enriching flower Elaeagnus angustifolia flavonoids. The opti- mal process conditions were investigated through single factor test. RESULTS DA201 resin Showed good adsorption and separation property for Elaeagnus angustifolia flavonoids. The detailed process conditions were as follows： the ratio of diameter to height of resin column was 1： 8, sample loading rate was 0. 5 mL · min^- 1, sample concentration was 20 mg· mL^- 1, pH was adjusted to 4, with elu-tion rate of 0. 5 mL ·min^-1, the largest sample load was 4. 5 BV, eluent concentration was 80% and the dosage was 12 BV. CON- CLUSION DA201 type resin, showed good purification effect for flavonoids Elaeagnus angustifolia in the defined process conditions with a yield of 85.23%. The purity of total flavonoids achieved 14. 95%, which was 3 times of that by coarse extraction （4. 901% ）.

关 键 词：沙枣花 总黄酮 静态吸附 动态吸附 大孔树脂 X-5树脂 HP-20树脂 

分 类 号：R284.2[医药卫生—中药学]