桦褐孔菌三萜对CCl_4致小鼠氧化应激损伤的保护作用  

Triterpenoids from Inonotus obliquus protect mice against oxidative damage induced by CCl_4

作　　者：赵芬琴[1,2] 严琳[3] 崔仙红[1] 林昇[1] 王聪[1] 张慧[1] 康晓燕[1] 姬汴生[3] 

机构地区：[1]河南大学药物研究所,河南开封475004 [2]沈阳药科大学中药学院,辽宁沈阳l 10016 [3]河南大学天然药物与免疫工程重点实验室,河南开封475004

出　　处：《药学学报》2012年第5期680-684,共5页Acta Pharmaceutica Sinica

基　　金：河南省天然药物与免疫工程重点实验室开放课题(KF201004)

摘　　要：桦褐孔菌（Inonotus obliquus）系多孔菌科药用真菌,主要分布于北纬45°～50°地区,在我国的黑龙江、吉林两省资源较丰富。民间广泛用于治疗一些恶性肿瘤[1]。已有大量现代药理学研究证明,桦褐孔菌具有抗肿瘤、抗炎、抗病毒、降血糖、To investigate the effects of lanosterol（1）,inotodiol（2） and trametenolic acid（3） from Inonotus obliquus against oxidative damage induced by CCl4 in mice,1,2 and 3（20,10 and 5 mg-kg-1） were respectively administered to mice,once a day for 3 days.Then the mice were induced to oxidative damage by CCl4 on the third day 30 min after the administration.The activities of superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-PX） and the content of malondialdehyde（MDA） and reductive glutathione（GSH） in serum and liver homogenate were determined.And the levels of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） and interleukin-6（IL-6） concentration in serum were detected.The results showed that treatment with compound 1,2 and 3 could significantly increase the activities of SOD,CAT and GSH-PX in serum and liver homogenate.Furthermore,the content of GSH in serum and liver homogenate increased and MDA content decreased markedly.In addition,compound 1,2 and 3 could significantly inhibit the activities of ALT and AST in serum,and decrease the IL-6 concentration in serum remarkably.So,compound 1,2 and 3 can protect mice against oxidative stress injury induced by CCl4.Furthermore,compound 1,2 and 3 can protect cells from damage through inhibition on ALT,AST and the expression of IL-6.

关 键 词：桦褐孔菌 三萜 羊毛甾醇 桦褐孔菌醇 栓菌酸 抗氧化作用 氧化应激 

分 类 号：R965[医药卫生—药理学]