白细胞介素7剪接变异体的克隆和序列分析  

Clone and analysis of splice variants of interleukin 7 in cancer cell lines

作　　者：冯凤兰[1] 彭羽瑜[1] 潘德顺[1] 李晓波 金小宝 朱家勇 

机构地区：[1]广东药学院药理系 [2]广东省生物活性药物研究重点实验室

出　　处：《中国临床药理学与治疗学》2012年第4期367-370,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：中国-意大利科技合作项目(200625)

摘　　要：目的:克隆白细胞介素-7(IL-7)剪接变异体,筛选出在IL-7开放阅读框内剪接变异体,为其生物活性的研究打基础。方法:运用自行设计的IL-7引物,采用逆转录聚合酶链反应(RT-PCR)技术,分离正常培养的肝癌细胞株HepG2、结肠癌细胞株HT29IL-7mRNA,再对电泳所获条带进行克隆、测序和分析。结果:肝癌细胞株HepG2、结肠癌细胞株HT29都能检测到IL-7mRNA,而且从培养癌细胞株分离出多条新带,经亚克隆及测序,证实这些条带是来源人IL-7基因、但缺乏不同外显子的IL-7剪接变异体cDNA;通过和IL-7cDNA比对分析,发现其中5个剪接变异体在IL-7开放阅读框内。结论:IL-7剪接变异体广泛存在于各种肿瘤细胞中。对其进一步的研究,将为癌症病人的免疫调节紊乱和临床免疫治疗提供新的研究方向。AIM： To clone and select the splice variants in open reading frame of interleukin 7 from cancer cell lines.METHODS： Reverse transcription-polymerase chain reaction（RT-PCR） was used for investigating the expression of IL-7.According to IL-7 gene cDNA,the primers were designed and synthesized,then the splice variants of IL-7 in human cancer cell lines was identified,cloned into vector and sequenced.RESULTS：IL-7 mRNA can be detected in Hepatic cancer cells and Colon cancer cells.A number of new bands of IL-7 were obtained from cultured cancer cell lines,which lack of different exons.By analyzing the splice variants,we found 5 of them in open reading frame of IL-7.CONCLUSION： Splice variants of IL-7 are expressed in many tumor cell lines.Further investigation of the Splice variants can provide a new research direction for cancer patient immune disorders and clinical immunotherapy.

关 键 词：白细胞介素7 克隆 序列分析 肿瘤细胞 剪接体 

分 类 号：R965.2[医药卫生—药理学]