电针对局灶性脑梗死大鼠神经功能及LINGO1表达的穴位比较研究  

A comparative study of acupoints by electroacupuncture on neural function and expression of LINGO1 after focal cerebral infarction in rats

作　　者：钟艺华[1,2] 李光勤[1] 唐显军[2] 黄浩然[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016 [2]重庆市肿瘤研究所神经内科

出　　处：《中国康复医学杂志》2012年第5期410-414,427,共6页Chinese Journal of Rehabilitation Medicine

摘　　要：目的:比较督脉取穴法(人中-百会穴)与督脉取穴法联合背俞取穴法(人中-百会穴+肝俞-肾俞穴)在局灶性脑缺血大鼠的治疗效果上是否存在差异。方法:将健康成年雄性SD大鼠91只随机分为4组:假手术组、模型组、治疗A组(电针人中-百会穴)、治疗B组(电针人中-百会穴+肝俞-肾俞穴),采用线栓法制作大鼠右侧大脑中动脉闭塞模型(MCAO)。电针刺激在造模成功90min后开始,30min/d。分别选取缺血第1、3、7及14天共4个时间点,运用Longa评分对神经功能恢复情况进行评价,RT-PCR及免疫组化测定缺血侧皮质LINGO1mRNA和蛋白表达。结果:假手术组无神经功能障碍。治疗A组和治疗B组运动功能恢复情况较模型组明显改善(P<0.05);LINGO1mRNA和蛋白表达在模型组中缺血第1天时明显增强第3—14天表达逐渐下降,但第14天时仍较假手术组高(P<0.01);治疗A组和治疗B组在第7—14天时其LINGO1mRNA和蛋白表达均较模型组低(P<0.05),并且,治疗B组在7—14天时的LINGO1蛋白表达较治疗A组低,二者之间差异有显著性意义(P<0.05)。结论:局灶性脑梗死大鼠缺血侧脑皮质LINGO1mRNA和蛋白表达明显上调,在脑梗死急性期给予电针刺激后能下调其表达水平,促进轴突再生,加速神经功能恢复;联合取穴法与单一取穴法比较,能取得更好的治疗效果。Objective： To investigate the difference between the effect of electroacupuncture （EA）at ＂Renzhong-Baihui＂ （DU26-DU20,RB） and ＂Renzhong-Baihui ＋ Ganshu-Shenshu＂ （DU26-DU20 ＋ BL 18-BL23, RB ＋ GS） after focal cerebral infarction in rats. Method： The healthy adult male SD rats （n=91） were randomly divided into sham-operated group, model group, treatment group A（RB）and treatment group B（RB＋GS） and subjected to establish the model of middle cerebral artery occlusion（MCAO）on the right side by nylon monofilament suture. EA was performed at 90 min after establishing of successful model, 30min/d. The neural function recovery was evaluated by Longa＇s score, RT-PCR and immunohistochemistry techniques were used to detect the expressions of LINGO1 mRNA and protein in ischemic cortex on the lst,3rd,Tth and 14th d respectively after ischemia. Result： The sham-operated group had no neurological dysfunction. Motor function recovered significantly in treatment group A and treatment group B, compared with model group（P〈0.05）. The expressions of LINGO1 mRNA and protein in model group enhanced obviously on the 1st d, then gradually decreased on the 3rd-14th d,but were still higher than that in sham-operated group on the 14th d（P〈0.01）. The expressions of LINGO1 mRNA and protein in treatment group A and treatment group B were all lower than that in model group on the 7d-14d （P〈0.05）;in addition,the expression of LINGO1 protein in treatment group B was lower than that in treatment group A,there was significant statistical difference between two treatment groups（P〈0.05）. Conclusion：The expressions of LINGO1 mRNA and protein in ischemic cortex increased significantly. EA might induce its＇ down-expression after acute cerebral infarction, promote axonal regeneration and accelerate the neural functional recovery. The therapeutic effect of RB＋GS group was better than that of RB group.

关 键 词：电针 脑梗死 LINGO1蛋白 轴突再生 

分 类 号：R245[医药卫生—针灸推拿学]