结核分枝杆菌抗原蛋白38×10~3抗原表位预测及其蛋白片段抗原性研究  

Epitope prediction of 38×10~3 from Mycobacterium tuberculosis and antigenicity analysis of related protein fragments

作　　者：石磊[1] 刘琪琦[2] 张敏丽[2] 陈苏红[2] 王升启[2] 

机构地区：[1]解放军总医院,北京100853 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学》2012年第4期276-279,共4页Military Medical Sciences

基　　金：国家传染病重大专项资助项目(2008ZX10003-009)

摘　　要：目的筛选出一种结核分枝杆菌(MTB)特异性诊断标志物。方法对MTB重组蛋白抗原38×103的基因组序列进行生物信息学分析及抗原表位预测,原核表达38×103基因序列121～182和238～374位氨基酸蛋白片段,分别命名为T38和P38重组蛋白;采用蛋白芯片对T38和P38重组蛋白进行抗原性检测。结果经蛋白芯片检测94例阳性和128例阴性样本,结果显示,P38重组蛋白在结核病检测中的敏感性为81.9%,特异性为80.5%,但是T38重组蛋白特异性不佳。结论生物信息学预测38×103基因抗原表位,为寻找有价值的抗原靶点提供了参考;重组蛋白片段P38在结核病诊断中具有潜在应用价值,可作为联合诊断的备选抗原之一。Objective To screen a specific diagnostic marker of Mycobacterium tuberculosis（MTB）.Methods The 38×103 epitope in MTB was analyzed and predicted,and the 121-182 and 238-374 amino acid fragments of 38×103 in prokaryotic vectors were named T38 and P38 recombinant proteins;their antigenicity was detected by protein chips.Results The protein chip was applied to detect 94 positive and 128 negative samples.The results showed certain sensitivity（81.9%） and high specificity（80.5%） when P38 recombinant protein was used as an antigen for tuberculosis diagnosis,but T38 had low specificity.Conclusion Bioinformatics is applied to predict the 38×103 epitope,so that a better target site of antigens can be fourd.P38 recombinant protein can be potentially used for the diagnosis of tuberculosis and as a candidate antigen.

关 键 词：结核分枝杆菌 蛋白片段 抗原性 蛋白芯片 

分 类 号：R392.1[医药卫生—免疫学]