束缚应激对大鼠免疫功能的影响  

Effect of restraint stress on rats′immune system

作　　者：王瑜[1,2] 赵云[1] 杲修杰[1] 万帝[3] 石卿[3] 张岭[3] 潘勤 钱令嘉[1] 

机构地区：[1]军事医学科学院基础医学研究所,全军军事认知与心理卫生研究中心,北京100850 [2]天津中医药大学研究生院,天津300193 [3]河北联合大学,河北唐山063009 [4]天津中新药业股份有限公司,天津300193

出　　处：《军事医学》2012年第4期289-290,294,共3页Military Medical Sciences

基　　金：国家973计划"肝藏血主疏泄现代科学内涵的实验研究"(2011CB505106)

摘　　要：目的探讨束缚应激对大鼠免疫功能的影响。方法大鼠随机分为4组:正常对照组(Ctr)、束缚1周组(R1)、束缚2周组(R2)和束缚4周组(R4)。取大鼠血清用ELISA方法检测糖皮质激素(GC)、白介素-6(IL-6)和白介素-17(IL-17)水平,单向免疫扩散法检测免疫球蛋白G(IgG)水平。分离外周血中单核细胞,用吞噬中性红法检测大鼠单核巨噬细胞(Mo/Mφ)的吞噬能力。结果束缚组大鼠GC、IL-6水平显著高于Ctr组,IL-17、IgG含量和外周血单核巨噬细胞吞噬能力显著低于Ctr组。结论束缚应激可以使大鼠免疫功能显著降低。Objective To explore the effect of restraint stress on the rat immune system.Methods Rats were randomly divided into four groups： normal control group（Ctr）,restraint for one week（R1）,restraint for two weeks（R2） and restraint for four weeks（R4）.Glucocorticoid（GC）,interleukin-6（IL-6） and interleukin-17（IL-17） in serum were determined by ELISA and immunoglobulin G（IgG） was determined by single radial immunodiffusion.The mononuclear cells were separated from the peripheral blood.The phagocytic activity of rat mononuclear macrophages（Mo/Mφ） was detected by the neutral red.Results GC and IL-6 levels in restraint rats were significantly higher than in the Ctr group while the contents of IL-17,IgG,and the phagocytic capacity of peripheral blood mononuclear cells were significantly lower than those of the Ctr group.Conclusion Restraint stress can significantly reduce the immune function of rats.

关 键 词：束缚 应激 免疫 

分 类 号：R392[医药卫生—免疫学]