大气PM2.5致人支气管上皮细胞DNA损伤的研究  

PM2.5-induced DNA Damage in Human Bronchial Epithelial Cells

作　　者：覃辉艳[1,2] 彭晓武[3] 蒙智娟[1] 黄炯丽[1] 李琴[1] 杨晓波[1] 韦小敏[1] 邹云锋[1] 

机构地区：[1]广西医科大学公共卫生学院毒理学教研室,广西南宁530021 [2]广西区疾病预防控制中心 [3]环境保护部华南环境科学研究所

出　　处：《环境与健康杂志》2012年第5期391-393,共3页Journal of Environment and Health

基　　金：2007年国家环保公益性行业科研专项(200709004)

摘　　要：目的研究大气PM2.5对人支气管上皮细胞(16-HBE)DNA的损伤效应。方法将人支气管上皮细胞(16-HBE)分别暴露于8、16、32、64、128μg/ml的广州大气PM2.5 24、48、72 h,采用MTT法测定细胞存活率,彗星试验检测细胞DNA损伤水平。结果染毒24、48、72 h后,64、128μg/ml组细胞存活率均明显低于对照组(P<0.05),各染毒组细胞Olive尾矩均明显高于对照组(P<0.05);72 h各剂量组细胞存活率均低于24 h相应处理组(P<0.05),48、72 h各剂量组细胞Olive尾矩与24 h相比均明显增加(P<0.05);经多重线性回归分析,Olive尾矩(y)与染毒剂量(d)和染毒时间(t)的回归方程为y=-6.481+0.162 d+0.210t(R^2=0.842,P<0.05)。结论大气PM2.5可导致人支气管上皮细胞(16-HBE)DNA损伤,且损伤程度与染毒剂量、时间有一定关联。Objective To study the effect of airborne particulate matter PM2.5 on DNA damage in human bronchial epithelial cells（16-HBE）. Methods The human bronchial epithelial cells（16-HBE） were exposed to 8, 16, 32, 64, 128 μg/ml airborne particulate matter PM2.5 collected from Guangzhou. After 24,48 and 72 h of incubation, the cell survival was measured by MTT assay. DNA damage was detected with Comet assay. Results After 24, 48 and 72 h of treatment, compared with the negative control, the cell survival rates of 64, 128μg/ml groups decreased significantly （P〈0.05）, and the DNA Olive tail moment of all groups increased significantly （P〈0.05）. Compared with the 24 h group, the cell survival rates of all treated groups in 72 h decreased significantly （P〈0.05）, and the DNA Olive tail moment of all groups in 48 and 72 h increased significantly （P〈0.05）. The regression equation between the DNA Olive tail and dose-time is y=-6.481＋0.162 dose＋0.210 time （R2=0.842,P〈0.05）. Conclusion Airborne particulate matter PM2.5 can induce human bronchial epithelial cells （16-HBE） DNA damage in a dose-time dependent manner.

关 键 词：空气污染 PM2.5 人支气管上皮细胞 DNA损伤 

分 类 号：R122.2[医药卫生—环境卫生学]