SOCS-1和RIZ1基因启动子区CPG岛甲基化与胃癌发病的关系  

Promoter Region Methylation of SOCS-1 and RIZ1 Genes in Gastric Cancer

作　　者：李鹏飞[1] 王佳[1] 严滢滢[1] 冯靖宇[1] 任晓峰[1] 沈孝兵[1] 

机构地区：[1]东南大学公共卫生学院环境医学工程教育部重点实验室,江苏南京210009

出　　处：《环境与健康杂志》2012年第5期394-396,共3页Journal of Environment and Health

基　　金：国家自然科学基金(81172619);江苏省自然科学基金(BK2009283)

摘　　要：目的检测细胞因子信号转导抑制因子(SOCS-1)和Rb结合锌指结构基因(RIZI)启动子区甲基化状态,初步探讨其甲基化状态与胃癌发生的关系。方法收集南京市原发性胃癌患者96例为胃癌组,采用1:1病例-对照研究方法 ,随机选取非消化系统疾病、非肿瘤患者96例为对照组,采用甲基化特异性PCR(MSP)检测胃癌组及对照组外周血DNA的SOCS-1和RIZl基因基因启动子区甲基化状态。结果胃癌组SOCS-I(OR=4.845,P<0.001,95%CI:2.506～9.367)和RIZI基因(OR=3.338,P=0.01,95%CI:1.591～7.003)启动子区甲基化率均高于对照组,且有统计学意义。在胃癌组中,发现SOCS-1基因甲基化异常与年龄、性别、Lauren分型、胃癌发生部位、TNM分期等无统计学关联(P>0.05),而与肿瘤淋巴有无转移有一定关联(P=0.002);RIZI基因甲基化异常与年龄、性别、Lauren分型、胃癌发生部位、TNM分期、淋巴结转移均未见统计学关联(P>0.05)。结论 SOCS-1和RIZI基因启动子区异常甲基化可能具有肿瘤特异性,检测SOCS-1和RIZI基因甲基化状态对于胃癌的早期诊断和治疗有一定意义。Objective To understand the relationship between CPG island methylation of promoter of the suppressor of cytokine signaling-1 （SOCS-1） gene, RIZ1 gene and human gastric cancer. Methods Methylation-specific PCR（MSP） technique were used to detect the methylation status of promoter of SOCS-1 and RIZ1 among 96 cases of gastric cancer and a 1：1 control group of cancer-free and matched by age（±5 years） and gender. For every subject the blood sample was collected from for analyzing. Results The rate of SOCS-1 and RIZ1 gene promoter aberrant methylation in gastric cancer was significantly higher than corresponding controls, OR values were 4.845（P〈0.001,95%CI：2.506-9.367） for SOCS-1, and 3.338（P=0.01, 95%CI： 1.591-7.003） for RIZ1. SOCS-1 and RIZ1 methylation weren＇t significantly correlated with the gender or tumor size and invasion depth of gastric cancer. But SOCS-1 was significantly correlated with the lymphatic metastasis. Conclusion High frequent methylation of SOCS-1 and RIZ1 is mostly cancer specific and it may play a role in the development of gastric cancer, which may provide useful clues for the early diagnosis of gastric cancer.

关 键 词：胃癌 SOCS-1基因 RIZ1基因 甲基化 

分 类 号：R181.3[医药卫生—流行病学]