新型杀菌剂烯肟菌胺对斑马鱼胚胎发育毒性的研究  

Developmental Toxicity of SYP-1620 to Zebrafish Embryos

作　　者：杨红莲[1] 崔新仪[2] 闫峻[1] 刘焕亮[1] 张伟[1] 袭著革[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所天津市环境与食品安全风险监控技术重点实验室,天津300050 [2]天津农学院园艺系

出　　处：《环境与健康杂志》2012年第5期410-412,F0003,共4页Journal of Environment and Health

基　　金：国家自然科学基金(20907075)

摘　　要：目的探讨新型杀菌剂烯肟菌胺(SYP-1620)对斑马鱼胚胎发育的影响。方法采用经济合作与发展组织(OECD)推荐的斑马鱼胚胎发育方法 ,将受精后2 h内的斑马鱼鱼卵暴露于不同浓度梯度的烯肟菌胺(0.1、0.2、0.4、0.8、1.0 mg/L)及其混配制剂爱可(1.0、2.0、4.0、8.0、16.0 mg/L)。观察烯肟菌胺和爱可暴露对斑马鱼胚胎发育形态及孵化率、死亡率的影响。用线性拟合法计算斑马鱼胚胎发育到96 h的半致死浓度(LC_(50)。结果烯肟菌胺和爱可暴露主要造成斑马鱼胚胎发育迟缓、孵化率和存活率明显降低,同时,还出现了胚胎卵黄膜外凸、仔鱼心包水肿和尾巴弯曲等形态的畸形以及仔鱼存活时间缩短;斑马鱼胚胎暴露于烯肟菌胺原药和爱可96 h的LC_(50)分别为0.43和10.27 mg/L。结论烯肟菌胺对斑马鱼胚胎发育具有明显的毒性作用,提示其对生态环境具有潜在危害。Objective To study the effects of the fungicide SYP-1620 on the development of zebrafish embryos. Methods In the present study ,the fertilized eggs of zebrafish in 2 h were exposed to SYP-1620 at the doses of 0.1,0.2,0.4,0.8 and 1.0 mg/L respectively and exposed to the mixture of 10% SYP-1620 plus tebuconazole at the doses of 1.0,2.0,4.0,8.0 and 16.0 mg/L respectively using embryo development technique recommended by OECD. The effects of two fungicides exposure on the morphological development, hatching rates and mortality rates of zebrafish embryos were studied. The 96 h LCs0 for SYP-1620 and its mixture in zebrafish embryos toxicity was calculated by linear-fitting method. Results SYP-1620 and the mixture of SYP-1620 exposure reduced hatching rates,survival rates of zebrafish embryos and abnormal development including pericardial edema and spinal flection abnormalities of larvae. The 96 h LC50 for SYP-1620 and its mixture in zebrafish embryos toxicity were 0.43 and 10.27 mg/L respectively. Conclusion SYP-1620 presents adverse effects on embryo development of zebrafish, which presents potencial hazard to the ecological environment.

关 键 词：烯肟菌胺 斑马鱼 胚胎发育 

分 类 号：R994.6[医药卫生—毒理学]