化瘀止痛方外用对骨转移癌疼痛大鼠脊髓背角的影响  

Influence of Formula for Resolving Stasis and Relieving Pain on Pinal Cord Dorsal Horn of Rats with Bone Cancer Pain

作　　者：邓博[1,2] 贾立群[2] 蔡大勇[1] 谭煌英[2] 高福云[2] 潘琳[2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]中日友好医院,北京100029

出　　处：《中华中医药学刊》2012年第5期972-973,共2页Chinese Archives of Traditional Chinese Medicine

基　　金：国家"十一五"科技支撑计划资助项目(2008BAI53B023)

摘　　要：目的:研究化瘀止痛方外用对骨转移癌疼痛大鼠的镇痛作用及机制。方法:采用大鼠乳腺癌MRMT-1细胞,按Medhurst方法建立骨转移癌疼痛模型。以唑来膦酸为阳性对照,观察外用药对大鼠脊髓背角神经元原癌基因c-FOS蛋白表达、神经胶质细胞胶质细胞酸性蛋白(GFAP)表达的影响。结果:相对于假手术组,各手术组脊髓后角神经元c-FOS蛋白表达明显增高,GFAP染色阳性星形胶质细胞明显增生肥大。与模型组比较,脊髓背角c-FOS表达明显降低(P<0.05),GFAP表达变化改善。结论:外用中药对骨转移癌疼痛模型有明显镇痛作用,作用机制与拮抗伤害感受器并且抑制痛觉在脊髓水平的放大有关。Objective： Evaluate the anti——nociceptive effects of herbal medicine extraction in a rat model of bone cancer pain.Methods：Following Medhurst＇s method,a rat model of cancer-induced bone pain was established using the MRMT-1 cell line.The corresponding segments of the ipsilateral spinal cord were processed for c-fos staining and glial fibrillary acidic protein（GFAP）staining.Results：Compared to sham-operation group,c-fos and GFAP expression in the spinal cord dorsal horn of the model group was significantly increased.Compared to model group,treatment with herbal medicine extraction（ad us.ext.）significantly inhibited c-fos expression（P0.05）,GFAP expression was also attenuated.Conclusions：The herbal medicine extraction is an anti-nociceptive agent in a rat model of metastatic cancer pain.

关 键 词：骨转移癌疼痛 外治法 中药 MRMT-1细胞 

分 类 号：R285.5[医药卫生—中药学]