基于T-bet/GATA-3表达研究化痰祛瘀法对哮喘小鼠气道炎症及免疫失衡的影响  

Effect of Phlegm and Stasis Resolving Method on Airway Inflammation and Transcription Factors T-bet and GATA-3 in Mice with Asthma

作　　者：张新光[1,2] 赵毅涛[1,3] 白莉[1,2] 李利清[1,2] 虞坚尔[1,2] 

机构地区：[1]上海中医药大学附属市中医医院,上海200071 [2]上海市中医药研究院中医儿科研究所,上海200071 [3]广东省中医院儿科,广东广州510120

出　　处：《中华中医药学刊》2012年第5期974-977,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30873286;81173302);国家临床重点专科建设项目

摘　　要：目的:研究转录因子T-bet和GATA-3在哮喘模型小鼠疾病进展过程中的改变及其意义及其在Th1/Th2失衡中的作用,探讨化痰祛瘀法对支气管哮喘防治作用的机理。方法:通过卵蛋白致敏、激发建立支气管哮喘小鼠模型,于激发后7天和21天分批取材,用RT-PCR法检测肺组织匀浆中T-bet、GATA-3 mRNA表达。结果:激发7天后:与空白组比较:模型组T-bet明显降低,GATA-3明显升高,T-bet/GATA-3比值明显降低;两用药组T-bet及T-bet/GATA-3比值均明显降低,组间无明显差异。与模型组比较:两用药组T-bet均无明显变化,GATA-3均明显降低,T-bet/GATA-3比值均明显升高;组间无明显差异。哮喘激发21天后:与空白组比较:模型组T-bet明显降低,GATA-3明显升高,T-bet/GATA-3比值明显降低;两用药组T-bet及T-bet/GATA-3比值均明显降低。与模型组比较:地塞米松组T-bet无明显差异,GATA-3明显降低,T-bet/GATA-3比值明显升高;中药组T-bet明显升高,GATA-3明显降低,T-bet/GATA-3比值明显升高;两组间无明显差异。结论:BALB/c小鼠经OVA致敏及雾化激发后,使转录因子T-bet/GATA-3表达失衡,模型小鼠出现气道炎症及气道重建的相关表现,符合中医病机中的"痰瘀互结"。化痰祛瘀法对T-bet/GATA-3表达失衡具有双向纠偏的调节作用。对改善气道慢性炎症及气道重建方面,其疗效与地塞米松相近,但作用环节较对照组更为广泛,且随用药时间延长,有累加效应。Objectives：To understand the changes of transcription factors T-bet and GATA-3 in the disease progression of bronchial asthma and the roles of them in Th1/Th2 imbalance,and to explore the mechanism of phlegm-resolving and stasis-removing method in preventing and treating bronchial asthma.Methods：The asthmatic BALB/c mice model were established through sensitizing and activating by intra-peritoneal injection and inhalation of ovalbumin,lung homogenate was obtained after the 7th and the 21st day of activation respectively,T-bet and GATA-3 mRNA expressions were detected by RT-PCR.Results：After the 7th day of activation,compared with the normal group,T-bet of model group was decreased and GATA-3 increased significantly,T-bet/GATA-3 ratio was decreased;both T-bet and the T-bet/GATA-3 ratio of the two treated groups were significantly decreased without inter-group difference;compared with the model group,there was no changes in T-bet levels,but GATA-3 were decreased,T-bet/GATA-3 ratios were increased in both treated groups without inter-group difference.After the 21st day of activation,compared with the normal group,T-bet level of model group was decreased and GATA-3 level increased significantly,T-bet/GATA-3 ratio was decreased;both T-bet and the T-bet/GATA-3 ratio of the two treated groups were significantly decreased without inter-group difference;compared with the model group,there was no significant change in T-bet level of dexamethasone（DXM） group,while its GATA-3 level was obviously decreased,and the T-bet/GATA-3 ratio increased significantly;of PCF group,T-bet level was increased,GATA-3 level decreased,and the T-bet/GATA-3 ratio increased significantly.No inter-group difference was found between the two treated groups.Conclusions：After sensitized and activated by OVA there is imbalance of transcription factors T-bet and GATA-3 expression,and the airway inflammation and remodeling appeared,which is in accordance with the pathomechnism of ＂coagulation of phlegm and stasis＂ in Chinese medicine theo

关 键 词：支气管哮喘 化痰祛瘀 T-BET/GATA-3 TH1/TH2 气道炎症 

分 类 号：R285.5[医药卫生—中药学]