通心络超微粉以及7-硝基吲唑对脑缺血再灌注大鼠海马一氧化氮的影响  

Effects of Tongxinluo Ultramicro-Pulverization and 7-Nitroindazole on NO Concentration in Hippocampus of Rats with Transient Cerebral Ischemia and Reperfusion

作　　者：邰春娇[1] 蒋敏海[2] 

机构地区：[1]南京医科大学附属杭州医院神经内科,江苏南京310006 [2]杭州市第一人民医院神经内科,浙江杭州310006

出　　处：《中华中医药学刊》2012年第5期994-996,I0001,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家人事部留学回国人员科技活动择优基金资助项目(2003433);杭州市科技发展计划医学重点专科专病资助项目(2005633Q21)

摘　　要：目的:研究通心络超微粉以及7-硝基吲唑(7-Nitroindazole,7-NI)对脑缺血再灌注大鼠海马一氧化氮(NO)产生的影响。方法:大鼠前脑缺血采用四血管阻断法,实验分为生理盐水组、通心络超微粉组以及7-NI组。选择性NO测定电极测定NO的浓度。结果:通心络超微粉以及7-NI没有影响大鼠的血压和海马的流量,与对照组相比,均显著减少缺血再灌注时海马内NO的产生(均<0.001)。结论:通心络超微粉、7-NI可以通过抑制神经型一氧化氮合酶(nNOS)从而减少NO的产生而起到神经保护的作用。Objective：To investigate the efficacy of Tongxinluo ultramicro-pulverization and 7-Nitroindazole（7-NI）on nitric oxide（NO）concentration in rat hippocampus after transient cerebral ischemia and reperfusion.Methods：The model of cerebral ischemia was made by 4-vessel occlusion for 10min.NO concentration was measured directly by an NO-selective electrode method.Saline、Tongxinluo ultramicro-pulverization and 7-NI were administered intraperitoneally 20min before the onset of ischemial.Results：Both Tongxinluo and 7-NI did not affect the hippocampal blood flow and mean arterial blood pressure（MABP）,compared with saline group.Tongxinluo and 7-NI significantly reduced the NO production during post-ischemic early reperfusion（P0.001）.Conclusion：Tongxinluo ultramicro-pulverization and 7-Nitroindazole have protective effect on hippocampal neurons against cerebral ischemia reperfusion injury by reducing the activity of neuronal NO synthase（nNOS）or reducing the content of NO in hippocampus of rat.

关 键 词：通心络7-硝基吲哚 一氧化氮 脑缺血 

分 类 号：R285.5[医药卫生—中药学]