外源Ca^(2+)对土人参(Talinum paniculatum)种子萌发和幼苗生长的影响  

Effect of Exogenous Ca^(2+) on Seeds Germination and Seedling Growth of Talinum paniculatum

作　　者：江富成[1] 王希[1] 杨柳[1] 张习敏[1] 乙引[1] 

机构地区：[1]贵州师范大学贵州省植物生理与发育重点实验室,贵阳550001

出　　处：《种子》2012年第5期19-22,共4页Seed

基　　金：国家自然科学基金(编号:30460031);贵州省优秀科技教育人才省长专项资金(黔省专合字[2008]26号);贵州省科学技术厅贵州师范大学联合基金(黔科合J字LKS[2010]18号)

摘　　要：将无菌土人参种子接种在含0.001、0.01、0.1、1.0、10、30 mmol/L Ca2+的MS固体培养基中,光照恒温培养,测定种子发芽率、发芽时间,以及幼苗形态、生理指标。结果表明,当培养基Ca2+浓度为0.1 mmol/L时,种子萌发时间最短,平均叶片数最多,平均株高最高,分别为9 d、11.82片和4.28 cm;当培养基Ca2+浓度为1.0 mmol/L时,种子发芽率、Chl a含量、Chl b含量最高,依次为74%、1.00 mg/g和0.14 mg/g;当培养基Ca2+浓度为30 mmol/L时,水势急剧下降;随着培养基中Ca2+浓度的增加,土人参根系活力呈曾加趋势,培养基中的Ca2+浓度为1.0 mmol/L时,根系活力为1.67 mg/(g.h)。土人参生长的最佳钙离子浓度范围为0.01～1.0 mmol/L。Effect of exogenous Ca2＋ on seed germination and seedling growth of Talinum paniculatum were studied. The seeds of Talinum paniculatum were planted in the medium containing 0.001 mmol/L ,0. 01 mmol/ L,0.1 mmol/L, 1.0 mmol/L, 10 mmol/L, 30 mmol/L Ca2＋ , respectively. The germination rate, germination time, physiological and morphological index were measured respectively. The results showed that the germination rate, content of chlorophyll a, content of chlorophyll b were the highest at 9 th day, 11.82 piece of leaves and 4.28 cm height in the medium containing 1.0 mmol/L Ca2 ＋. The germination time was the shortest and the numbers of leaves was the most when the medium contained 0.1 mmol/L Ca2＋. The water potential decreased at 30 mmol/L Ca2＋ in the medium. The roots activity increased with the increasing of Ca2＋ concentration. The roots activity was 1.67 mg/（g, h） at 1.0 mmol/L in the medium. The optimum Ca2＋ concentration was 0.01 - 1.0 mmol/L.

关 键 词：外源CA2+ 土人参 种子萌发 幼苗生长 

分 类 号：S567.5[农业科学—中草药栽培]