UPLC-Q-TOF-MS测定治咳川贝枇杷滴丸中6种有效成分的含量  

Determination of Six Active Components in Zhikechuanbeipipa Dropping Pills by UPLC-Q-TOF

作　　者：董林毅[1] 张韻慧[1] 郭婷婷[2] 刘艳杰[2] 齐欣[3] 侯媛媛[2] 

机构地区：[1]天津大学药物科学与技术学院,天津300072 [2]南开大学药学院,天津300071 [3]天津中新药业集团股份有限公司第六中药厂,天津300401

出　　处：《中国药学杂志》2012年第10期830-833,共4页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81173638);国家自然基金青年科学基金资助项目(81001682)

摘　　要：目的建立同时测定治咳川贝枇杷滴丸中6种有效成分(鸟苷、贝母素乙、桔梗皂苷D、桔梗皂苷D3、齐墩果酸和熊果酸)含量的超高压液相色谱串联四级杆飞行时间质谱联用(UPLC-Q-TOF-MS)的检测方法。方法采用Waters Acquity BEHC18色谱柱(2.1 mm×100 mm,1.7μm),流动相为乙腈-甲酸-水体系梯度洗脱,流速为0.4 mL·min-1,PDA检测190～400 nm扫描;进样量:2.0μL。结果 6种成分在一定范围内均呈现良好线性,r均大于0.999 6;精密度、重复性和稳定性良好;加样回收率在99%～101%之间。结论所建立的方法简便、准确、快速、重复性好,可用于治咳川贝枇杷滴丸中鸟苷、贝母素乙、桔梗皂苷D、桔梗皂苷D3、齐墩果酸和熊果酸6个有效成分的含量测定。OBJECTIVE To develop a UPLC-Q-TOF-MS method for determination of six components （guanosine, peiminine, platycodin D, platycodin D3 , oleanolic acid and ursolic acid） in Zhikechuanbeipipa dropping pills . METHODS The analysis was performed on a Waters Acquity BEH C18 column （2. 1 mm × 100 mm, 1.7 μm）with the mixture of acetonitrile-water-formic acid as mobile phase, and the flow rate was 0.4 mL · min-1 ; The PDA detection wavelength was 190 -400 rim; The column temperature was room temperature（30℃ ）. RESULTS The linear relationship between the concentration and peak areas of the six compounds were all linear （r 〉 0. 999 6）. The average recoveries （n = 6） were between 99% - 101%. CONCLUSION This method was simple, accurate and reliable to determine the six contents of Zhikechuanbeipipa dropping pills for the quality control.

关 键 词：治咳川贝枇杷滴丸 超高压液相色谱 串联四级杆飞行时间质谱 贝母素乙 桔梗皂苷 齐墩果酸 

分 类 号：R917[医药卫生—药物分析学]