不同栽培条件下白花丹根、茎、叶中白花丹醌的含量测定  

Determination of Plumbago Content in Herba Plumbaginis Zeylanicae under Different Growing Conditions

作　　者：戴先芝[1,2] 李艳丹[2] 黄艳菲[2] 丁玲[2] 张吉仲[2] 任朝琴[1] 刘圆[2] 

机构地区：[1]阿坝师范高等专科学校,四川汶川623002 [2]西南民族大学民族医药研究院,成都610041

出　　处：《中国药学杂志》2012年第10期834-837,共4页Chinese Pharmaceutical Journal

基　　金：国家科技支撑计划(2011SZ0233);四川省杰出青年学术技术带头人后续计划(2011JQ0051);四川省科技支撑计划(2011SZ0233);中央高校基本科研业务费专项资金项目优秀科研团队及重大孵化项目(11NZYTH02)

摘　　要：目的对家种不同栽培条件下白花丹药材根、茎、叶中的白花丹醌进行含量测定,为建立白花丹野生变家种的栽培技术规范提供实验数据。方法采用高效液相色谱法测定家种药材根、茎、叶中白花丹醌的含量。结果在熟土和黏土中生长的药材中白花丹醌的含量高于沙土、生土;施加K肥和N肥的药材中白花丹醌的含量高于施加P肥的药材,在全光照条件下生长的药材白花丹醌含量最高;不同繁殖方式中,根、茎和叶中白花丹醌的含量变化不大。结论课题组前期研究成果表明,白花丹醌为白花丹药材抗癌和抗炎等生物活性的主要有效成分,因此,从白花丹醌含量的角度考虑,白花丹药材的最佳栽培条件为:土壤为黏土,采用扦插苗,生长过程中施加一定的N肥和K肥,光照条件为全光照,在此条件下可获得优质高产的白花丹药材。OBJECTIVE To determine Plumbago content in Herba Plumbaginis Zeylanicae under different growing conditions and to provide experimental data for its domestication. METHODS High performance liquid chromatography was used to determine Plumbago contents in the roots, stems and leaves of home-grown Herba Plumbaginis Zeylanicae. RESULTS Plumbago content in Herba Plumbaginis Zeylanicae grown in mellow and clay soil was higher than in those grown in sand and crude soil. Potasium-fertilized and ni- trogen-fertilized Herba Plumbaginis Zeylanicae contained higher Plumbago than those fertilized by phosphorus. Plumbago content was the highest in Herba Plumbaginis Zeylanicae grown in full light. Plumbago contents in roots, stems, and leaves of Herba Plumbaginis Zeylanicae grown under different bred conditions did not vary much. CONCLUSION The research group＇s preliminary studies indi- cated that Plumbago was the main effective ingredient having anti-cancer and anti-inflammation bioactivity. Clay soil, cuttings, potassium fertilizer and nitrogen fertilizer, and full light were thought as ideal conditions for increasing Plumbago content. High quality Herba Plumbaginis Zeylanicae can be grown under these conditions.

关 键 词：栽培条件 白花丹 白花丹醌 高效液相色谱法 

分 类 号：R282[医药卫生—中药学]