高黏附力双歧杆菌的筛选与鉴定  

Screening and Identification of high adhesion Bifidobacteria to HT-29 Cell

作　　者：万翠香[1,2] 章昭琳[1] 王报贵[1] 魏华[1,2] 甘艳云 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047 [2]南昌大学中德联合研究院,南昌330047 [3]江西省新余市农业局,江西新余338000

出　　处：《中国乳品工业》2012年第5期13-15,33,共4页China Dairy Industry

基　　金：国家自然科学基金项目(30900038);国家“863”计划专题(2008AA10Z337);国家自然科学基金项目(31000048);2009年江西省主要学科学术和技术带头人培养计划;江西省教育厅科研基金(GJJ10379)

摘　　要：利用体外细胞培养法,从实验室现有的10株双歧杆菌中筛选具有较强粘附能力的菌株。采用革兰氏染色法和平板计数法,评价了这10株双歧杆菌对HT-29细胞的粘附性能,通过测定这10株双歧杆菌的16S rRNA基因序列(16S rDNA)进行分类学鉴定。结果显示,WBBI01,WBBI02,WBIN03,WBBI06具有极强的黏附力,其黏附指数分别达1.97×103,2.17×103,3.57×103,1.88×103。经16S rDNA测序鉴定WBBI01,WBIN03,WBBI06均与两歧双歧杆菌S17有极高的同源性,而WBBI02属于长双歧杆菌。结果表明,双歧杆菌黏附性能具有明显的种属特异性,不同种属双歧杆菌的粘附能力相差极大,以两歧双歧杆菌的黏附性能最强,婴儿长双歧杆菌为最弱。The adhesion capacity of ten Bifidobacteria strains in vitro was investigated. Gram staining and plate counting method were used to evaluate their adhesion capacity to HT-29 cell, and 16S rDNA sequencing analysis was carried out to identify the strain.. Based on the data in vitro, High attachment was observed in Bifidobacteria WBBI01, WBBI02, WBIN03, WBBI06, their adhesion index reached （bacterial ceUs/100 Caco-2 cells） 1.97x 103,2.17x10^3,3.57x10^3,1.88x10^3 repectively, while low attachment was shown in other strains. Identification of the strain indicates that the homology of Bifidobacteria WBBI01, WBIN03, WBBI06 with B. bifidum S17 were all above 99%, WBBI02 belongs to B. longum. The adhesion capacity varied considerably among different strains. B. bifidum WBIN03 was the most adhesive and the adhesion ability of Bifidobacteria WBAN07 was the weakest among all the tested strains.

关 键 词：益生菌 16S RDNA 黏附 双歧杆菌 黏附指数 

分 类 号：Q93-33[生物学—微生物学]